Distribution of CGRP and its receptor components CLR and RAMP1 in the rat retina

被引：23

作者：

Blixt, Frank W. ^{[1
]}

Radziwon-Balicka, Aneta ^{[2
]}

Edvinsson, Lars ^{[1
,2
]}

Warfvinge, Karin ^{[1
,2
]}

机构：

[1] Lund Univ, Dept Clin Sci, Div Expt Vasc Res, Lund, Sweden

[2] Rigshospitalet, Glostrup Res Inst, Dept Clin Expt Res, Glostrup, Denmark

来源：

EXPERIMENTAL EYE RESEARCH | 2017年 / 161卷

基金：

瑞典研究理事会;

关键词：

Immunohistochemistry; Flow cytometry; Retina; CGRP; CLR; RAMP1; GENE-RELATED PEPTIDE; ENDOTHELIAL-CELLS; MIGRAINE; PATHOPHYSIOLOGY; LIGHT; ADRENOMEDULLIN;

D O I：

10.1016/j.exer.2017.06.002

中图分类号：

R77 [眼科学];

学科分类号：

100212 ;

摘要：

Calcitonin gene-related peptide (CGRP) is a 37 amino acid neuropeptide with several functions including vasodilation, the perception of painful stimuli, and inflammation. The CGRP receptor consists of two main components; calcitonin-like receptor (CLR) and receptor activity-modifying protein 1 (RAMP1). While there is a growing recognition that CGRP plays a key role in migraine, the function of CGRP in the retina has not been fully established. This study aims to investigate the distribution of CGRP and its two receptor components in the rat retina, visually by immunohistochemistry and quantitatively using flow cytometry. CGRP immunoreactivity was found in the Muller cells while CLR/RAMP1 was located in the nerve fiber layer. Furthermore, since almost all RAMP1 immunoreactive cells co-express CLR, we propose that RAMP1 expression in the retina reflects functional CGRP receptors. (C) 2017 Elsevier Ltd. All rights reserved.

引用

页码：124 / 131

页数：8

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