Assay of 1-hydroxypyrene via aggregation-induced quenching of the fluorescence of protamine-modified gold nanoclusters and 9-hydroxyphenanthrene-based sensitization

被引：2

作者：

Xue, Jin-Hua ^{[1
]}

Liu, Ling ^{[1
]}

Wang, Yong-Sheng ^{[1
]}

Li, Jia-Qian ^{[1
]}

Li, Mei ^{[1
]}

Qu, Yue-Ning ^{[1
]}

Li, Le ^{[1
]}

机构：

[1] Univ South China, Coll Publ Hlth, Hengyang 421001, Peoples R China

来源：

MICROCHIMICA ACTA | 2019年 / 186卷 / 11期

基金：

中国国家自然科学基金;

关键词：

Nanoprobe; Photoluminescence quenching; High quantum efficiency; Electrostatic interactions; Hydrogen bonding; Hydrophobic interaction; Resonance energy transfer; Human urine samples; INDUCED EMISSION ENHANCEMENT; URINARY; 1-HYDROXYPYRENE; ACRIDINE-ORANGE; ENERGY-TRANSFER; CHROMATOGRAPHY; MICROEXTRACTION; NANOPARTICLES; 2-NAPHTHOL; EXPOSURE; SENSOR;

D O I：

10.1007/s00604-019-3810-1

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

This work describes a method for the determination of 1-hydroxypyrene (OH-Py) via aggregation-induced quenching of the emission of protamine-coated gold nanoclusters using 9-hydroxyphenanthrene (OH-Phe) as a sensitizer to boost the emission efficiency of nanoprobe. Under optimum conditions, the drop in fluorescence intensity at excitation/emission wavelengths of 300/596 nm is proportional to the concentrations of OH-Py in the range from 1.0 to 65 nM. The relative standard deviations are 4.2, 2.4 and 1.9% (for n = 11) at concentration levels of 8.0, 32 and 48 nM of OH-Py, respectively. The detection limit is 0.3 nM which is much lower than that of some previously reported methods. The recoveries from urine samples spiked with OH-Py ranged between 94.4 and 98.8%.

引用

页数：9

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