Regulation of axonal trafficking of cytochrome c oxidase IV mRNA

被引：56

作者：

Aschrafi, Armaz ^{[1
]}

Natera-Naranjo, Orlangie ^{[1
]}

Gioio, Anthony E. ^{[1
]}

Kaplan, Barry B. ^{[1
]}

机构：

[1] NIMH, Mol Biol Lab, NIH, Bethesda, MD 20892 USA

来源：

MOLECULAR AND CELLULAR NEUROSCIENCE | 2010年 / 43卷 / 04期

关键词：

Sympathetic neurons; Superior cervical ganglion; Intra-axonal translation; mRNA localization; 3 ' UTR; Axonal growth; Mitochondria; ATP synthesis; LOCAL PROTEIN-SYNTHESIS; 3'-UNTRANSLATED REGION; MITOCHONDRIA; LOCALIZATION; TRANSPORT; VICINITY; YEAST; HYBRIDIZATION; DENDRITES; ELEMENT;

D O I：

10.1016/j.mcn.2010.01.009

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Trafficking and local translation of axonal mRNAs play a critical role in the development and function of this neuronal subcellular structural domain. In this report, we studied cytochrome c oxidase subunit IV (COXIV) mRNA trafficking into distal axons of primary superior cervical ganglia (SCG) neurons, and provided evidence that axonal trafficking and mitochondrial association of the mRNA are mediated by an element located in a 38 bp-long, hairpin-loop forming region within the 3'UTR of the transcript. Our results also suggest that suppression of local translation of COXIV mRNA results in significant attenuation of axonal elongation. Taken together, the results provide the first evidence for the existence of a cis-acting axonal transport element within a nuclear-encoding mitochondrial gene, and demonstrate the importance of the axonal trafficking and local translation of nuclear-encoded mitochondrial mRNAs in axonal growth. Published by Elsevier Inc.

引用

页码：422 / 430

页数：9

共 36 条

[1] Mitochondrial biogenesis in the axons of vertebrate peripheral neurons [J].