Integrated analysis identified NPNT as a potential key regulator in tumor metastasis of hepatocellular carcinoma

被引:8
作者
Huang, Lingkun [1 ]
Guan, Shuzhen [1 ]
Feng, Lin [2 ]
Wei, Jinrui [3 ]
Wu, Lichuan [1 ]
机构
[1] Guangxi Univ, Med Coll, Nanning 530004, Peoples R China
[2] Peoples Liberat Army Gen Hosp, Dept Pathol, Med Ctr 1, Beijing, Peoples R China
[3] Guangxi Univ Chinese Med, Guangxi Sci Res Ctr Tradit Chinese Med, Nanning, Guangxi, Peoples R China
关键词
Hepatocellular carcinoma; Metastasis; WGCNA; NPNT; COEXPRESSION NETWORK ANALYSIS; BREAST-CANCER; LIVER; GENE; MIGRATION; BIOMARKER; ADHESION;
D O I
10.1016/j.gene.2022.146436
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Hepatocellular carcinoma (HCC) is one of the lethal malignancies worldwide. Tumor metastasis is the main cause of HCC related death. Although progress has been made in the mechanism study of HCC in the past decades, the underlying mechanism of HCC metastasis has not been fully illustrated. In the present study, bioinformatic analysis including weighted gene co-expression network analysis (WGCNA), differentially expressed gene analysis, and gene enrichment analysis were applied to discover genes correlated with HCC metastasis. Immunohistochemistry (IHC) assays were applied to detect the expression of NPNT in HCC samples. Cell transfection, wound healing, matrigel transwell assays, and western blot assays were utilized to evaluate the effects of NPNT on cell migration and invasion and signaling pathway variation. We found that NPNT was up-regulated in HCC tumor tissues compared with normal tissues. Especially, NPNT was highly expressed in metastatic tumor compared with non-metastatic HCC tumors. Down-regulation of NPNT via siRNA transfection inhibited cell migration, invasion, and FAK/PI3K/AKT signaling pathway in HCC. Our results demonstrate that NPNT is a potential key regulator in HCC metastasis.
引用
收藏
页数:8
相关论文
共 50 条
  • [21] Analysis of potential key genes in very early hepatocellular carcinoma
    Min Wu
    Zhaobo Liu
    Xin Li
    Aiying Zhang
    Dongdong Lin
    Ning Li
    World Journal of Surgical Oncology, 17
  • [22] ANGPTL2 promotes tumor metastasis in hepatocellular carcinoma
    Gao, Lin
    Ge, Chao
    Fang, Tao
    Zhao, Fangyu
    Chen, Taoyang
    Yao, Ming
    Li, Jinjun
    Li, Hong
    JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY, 2015, 30 (02) : 396 - 404
  • [23] Aberrant methylation status of SPG20 promoter in hepatocellular carcinoma: A potential tumor metastasis biomarker
    He, Lifeng
    Fan, Xiaoxiao
    Li, Yirun
    Cui, Bin
    Shi, Zhaoqi
    Zhou, Daizhan
    Lin, Hui
    CANCER GENETICS, 2019, 233 : 48 - 55
  • [24] FNBP4 is a Potential Biomarker Associated with Cuproptosis and Promotes Tumor Progression in Hepatocellular Carcinoma
    Zheng, Kai-Wen
    Zhang, Chao-Hua
    Wu, Wu
    Zhu, Zhu
    Gong, Jian-Ping
    Li, Chun-Ming
    INTERNATIONAL JOURNAL OF GENERAL MEDICINE, 2023, 16 : 467 - 480
  • [25] Potential Role of microRNA-183 as a Tumor Suppressor in Hepatocellular Carcinoma
    Bian, Wei
    Zhang, Hongfei
    Tang, Miao
    Zhang, Shaojun
    Wang, Lichao
    Liu, Longlong
    Wang, Wenyao
    CELLULAR PHYSIOLOGY AND BIOCHEMISTRY, 2018, 51 (05) : 2065 - 2072
  • [26] FNDC3B promotes cell migration and tumor metastasis in hepatocellular carcinoma
    Lin, Chin-Hui
    Lin, Yao-Wen
    Chen, Ying-Chun
    Liao, Chen-Chung
    Jou, Yuh-Shan
    Hsu, Ming-Ta
    Chen, Chian-Feng
    ONCOTARGET, 2016, 7 (31) : 49498 - 49508
  • [27] Midkine promotes hepatocellular carcinoma metastasis by elevating anoikis resistance of circulating tumor cells
    Sun, Bin
    Hu, Congli
    Yang, Zhibin
    Zhang, Xiaofeng
    Zhao, Linlin
    Xiong, Junye
    Ma, Junyong
    Chen, Lei
    Qian, Haihua
    Luo, Xiangji
    Shi, Lehua
    Li, Jun
    Cheng, Xianshuo
    Yin, Zhengfeng
    ONCOTARGET, 2017, 8 (20) : 32523 - 32535
  • [28] Suppression of hepatic tumor growth and metastasis by metronomic therapy in a rat model of hepatocellular carcinoma
    Jang, Jeong Won
    Park, Seong Tae
    Kwon, Jung Hyun
    You, Chan Ran
    Choi, Jong Young
    Jung, Chan-Kwon
    Bae, Si Hyun
    Yoon, Seung Kew
    EXPERIMENTAL AND MOLECULAR MEDICINE, 2011, 43 (05) : 305 - 312
  • [29] Downregulation of ZEB2-AS1 decreased tumor growth and metastasis in hepatocellular carcinoma
    Lan, Tian
    Chang, Lei
    Wu, Long
    Yuan, Yufeng
    MOLECULAR MEDICINE REPORTS, 2016, 14 (05) : 4606 - 4612
  • [30] Potential Prognostic Biomarkers for Bone Metastasis from Hepatocellular Carcinoma
    Xiang, Zuo-Lin
    Zeng, Zhao-Chong
    Tang, Zhao-You
    Fan, Jia
    He, Jian
    Zeng, Hai-Ying
    Zhu, Xiao-Dong
    ONCOLOGIST, 2011, 16 (07) : 1028 - 1039