Upconversion Nanoparticles and Monodispersed Magnetic Polystyrene Microsphere Based Fluorescence Immunoassay for the Detection of Sulfaquinoxaline in Animal-Derived Foods

被引：76
作者：
Hu, Gaoshuang [1 ]
Sheng, Wei [1 ,2 ]
Zhang, Yan [1 ,2 ]
Wang, Junping [1 ,2 ]
Wu, Xuening [1 ]
Wang, Shuo [1 ,2 ]
机构：
[1] Tianjin Univ Sci & Technol, Key Lab Food Nutr & Safety, Minist Educ China, Tianjin 300457, Peoples R China
[2] Tianjin Food Safety & Low Carbon Mfg Collaborat I, Tianjin 300457, Peoples R China
来源：
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2016年 / 64卷 / 19期
基金：
中国国家自然科学基金;
关键词：
SQX; UCNPs; MMPMs; fluorescence immunoassay; animal-derived foods; LINKED-IMMUNOSORBENT-ASSAY; PERFORMANCE LIQUID-CHROMATOGRAPHY; TANDEM MASS-SPECTROMETRY; SULFONAMIDE RESIDUES; CHICKEN MUSCLE; SAMPLES; MILK; ELISA; TISSUES; EGGS;
D O I：
10.1021/acs.jafc.6b01497
中图分类号：
S [农业科学];
学科分类号：
09 ;
摘要：
A novel fluorescence immunoassay for detecting sulfaquinoxaline (SQX) in animal-derived foods was developed using NaYF4:Yb/Tm upconversion nanoparticles (UCNPs) conjugated with antibodies as fluorescence signal probes, and monodisperse magnetic polystyrene microspheres (M.MPMs) modified with coating antigen as immune-sensing capture probes for trapping and separating the signal probes. Based on a competitive immunoassay format, the detection limit of the proposed method for detecting SQX was 0.1 mu g L-1 in buffer and 0.5 mu g kg(-1) in food samples. The recoveries of SQX in spiked samples ranged from 69.80 to 133.00%, with coefficients of variation of 0.24-25.06%. The extraction procedure was fast, simple, and environmentally friendly, requiring no organic solvents. In particular, milk samples can be analyzed directly after simple dilution. This method has appealing properties, such as sensitive fluorescence response, a simple and fast extraction procedure, and environmental friendliness, and could be applied to detecting SQX in animal-derived foods.
引用
收藏
页码：3908 / 3915
页数：8
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