Impact of combined NO and PG blockade on rapid vasodilation in a forearm mild-to-moderate exercise transition in humans

被引:34
作者
Saunders, NR
Dinenno, FA
Pyke, KE
Rogers, AM
Tschakovsky, ME
机构
[1] Queens Univ, Sch Phys & Hlth Educ, Kingston, ON K7L 3N6, Canada
[2] Mayo Clin, Dept Anesthesiol, Rochester, MN USA
[3] Mayo Clin, Gen Clin Res Ctr, Rochester, MN USA
来源
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY | 2005年 / 288卷 / 01期
关键词
muscle blood flow; vascular conductance; Doppler ultrasound;
D O I
10.1152/ajpheart.00762.2004
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
We tested the hypothesis that nitric oxide ( NO) and prostaglandins (PGs) contribute to the rapid vasodilation that accompanies a transition from mild to moderate exercise. Nine healthy volunteers ( 2 women and 7 men) lay supine with forearm at heart level. Subjects were instrumented for continuous brachial artery infusion of saline ( control condition) or combined infusion of N-G-nitro-L-arginine methyl ester (L-NAME) and ketorolac ( drug condition) to inhibit NO synthase and cyclooxygenase, respectively. A step increase from 5 min of steady-state mild (5.4 kg) rhythmic, dynamic forearm handgrip exercise (1 s of contraction followed by 2 s of relaxation) to moderate (10.9 kg) exercise for 30 s was performed. Steady-state forearm blood flow (FBF; Doppler ultrasound) and forearm vascular conductance (FVC) were attenuated in drug compared with saline (control) treatment: FBF = 196.8 +/- 30.8 vs. 281.4 +/- 34.3 ml/min and FVC = 179.3 +/- 29.4 vs. 277.8 +/- 34.8 ml.min(-1).100 mmHg(-1) (both P < 0.01). FBF and FVC increased from steady state after release of the initial contraction at the higher workload in saline and drug conditions: Delta FBF = 72.4 +/- 8.7 and 52.9 +/- 7.8 ml/min, respectively, and Delta FVC = 66.3 +/- 7.3 and 44.1 +/- 7.0 ml.min(-1).100 mmHg(-1), respectively (all P < 0.05). The percent DeltaFBF and DeltaFVC were not different during saline infusion or combined inhibition of NO and PGs: DeltaFBF = 27.2 +/- 3.1 and 28.1 +/- 3.8%, respectively (P = 0.78) and DeltaFVC = 25.7 +/- 3.2 and 26.0 +/- 4.0%, respectively (P = 0.94). The data suggest that NO and vasodilatory PGs are not obligatory for rapid vasodilation at the onset of a step increase from mild- to moderate-intensity forearm exercise. Additional vasodilatory mechanisms not dependent on NO and PG release contribute to the immediate and early increase in blood flow in an exercise-to-exercise transition.
引用
收藏
页码:H214 / H220
页数:7
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