Genetic parameters for tolerance to gill-associated virus under challenge-test conditions in the black tiger shrimp (Penaeus monodon)

被引:14
作者
Noble, Tansyn H. [1 ,2 ,3 ]
Coman, Gregory J. [1 ,3 ]
Wade, Nicholas M. [1 ,3 ]
Thomson, Peter C. [1 ,5 ]
Raadsma, Herman W. [1 ,4 ]
Khatkar, Mehar S. [1 ,4 ]
Guppy, Jarrod L. [1 ,2 ]
Jerry, Dean R. [1 ,2 ]
机构
[1] James Cook Univ, Australian Res Council Ind Transformat Hub Adv Pr, Townsville, Qld, Australia
[2] James Cook Univ, Coll Sci & Engn, Townsville, Qld, Australia
[3] Queensland Biosci Precinct, CSIRO Agr & Food, Aquaculture Program, St Lucia, Qld, Australia
[4] Univ Sydney, Fac Sci, Sydney Sch Vet Sci, Camden, NSW, Australia
[5] Univ Sydney, Fac Sci, Sch Life & Environm Sci, Camden, NSW, Australia
基金
澳大利亚研究理事会;
关键词
Disease tolerance; Heritability; Selective breeding; Gill-associated virus; Challenge test; Black tiger shrimp; TAURA SYNDROME VIRUS; LITOPENAEUS-VANNAMEI; CRASSOSTREA-GIGAS; RESISTANCE; INFECTION; INCREASES; SURVIVAL; LOADS; WSSV; AQUACULTURE;
D O I
10.1016/j.aquaculture.2019.734428
中图分类号
S9 [水产、渔业];
学科分类号
0908 ;
摘要
Diseases in shrimp aquaculture cause significant economic losses, particularly those caused by viruses. Genetic improvement of disease tolerance through selective breeding can be an important tool for managing the risk of disease in shrimp farming as long as the trait has a genetic basis (i.e. heritable) and is able to be accurately and reliably measured. This study presents the first estimates of genetic variation associated with tolerance to gill-associated virus (GAV) in Penaeus monodon using a unique controlled challenge WA design. Shrimp families (n = 72) were communally spawned and reared prior to replicated communal challenge testing when they reached an average size of similar to 8 g. Pedigree determination was carried out using genotyping and shrimp were challenged by intramuscular injection with a weight standardised dose of GAV. Daily mortality data were collected over 15 days from 1717 individuals and GAV infection load was measured on the challenge WA survivors (n = 963). Overall mortality after 15 days was 35.5% and ranged from 0 to 71% among families. Heritability estimates for mortality were 0.11 +/- 0.03 using a binomial model and 0.14 (standard error not estimated) using Cox's proportional hazards model. The estimated heritability for GAV infection load was 0.21 +/- 0.07. The genetic correlation between mortality and GAV infection load was not significantly different from zero, r(g) = 0.30 +/- 0.23, suggesting that the two traits may be genetically independent, at least in the way they were measured in this study. Results from this study suggest a low but significant genetic basis for GAV tolerance in P. monodon and that selection may be a viable option to manage GAV-related disease. The study also demonstrated, for the first time in shrimp, communal-based spawning and rearing can be used for large-scale genetic analysis of disease tolerance, eliminating the need for single family rearing tanks and associated confounding environmental effects.
引用
收藏
页数:9
相关论文
共 57 条
  • [1] Facts, truths and myths about SPF shrimp in Aquaculture
    Alday-Sanz, Victoria
    Brock, James
    Flegel, Timothy W.
    McIntosh, Robins
    Bondad-Reantaso, Melba G.
    Salazar, Marcela
    Subasinghe, Rohana
    [J]. REVIEWS IN AQUACULTURE, 2020, 12 (01) : 76 - 84
  • [2] Low viral loads and lymphoid organ spheroids are associated with yellow head virus (YHV) tolerance in whiteleg shrimp Penaeus vannamei
    Anantasomboon, Gun
    Poonkhum, Raksawan
    Sittidilokratna, Nusara
    Flegel, Timothy W.
    Withyachumnarnkul, Boonsirm
    [J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY, 2008, 32 (06) : 613 - 626
  • [3] [Anonymous], [No title captured]
  • [4] [Anonymous], [No title captured]
  • [5] [Anonymous], 2019, THESIS J COOL U TOWN
  • [6] [Anonymous], [No title captured]
  • [7] [Anonymous], [No title captured]
  • [8] [Anonymous], 2017, ASReml-R reference manual version 4, DOI DOI 10.15672/HJMS.2017.417
  • [9] Selective breeding of Pacific white shrimp (Litopenaeus vannamei) for growth and resistance to Taura Syndrome Virus
    Argue, BJ
    Arce, SM
    Lotz, JM
    Moss, SM
    [J]. AQUACULTURE, 2002, 204 (3-4) : 447 - 460
  • [10] Genomic predictions can accelerate selection for resistance against Piscirickettsia salmonis in Atlantic salmon (Salmo salar)
    Bangera, Rama
    Correa, Katharina
    Lhorente, Jean P.
    Figueroa, Rene
    Yanez, Jose M.
    [J]. BMC GENOMICS, 2017, 18