Momordica Charantia Polysaccharides Attenuates MPP+-Induced Injury in Parkinson's Disease Mice and Cell Models by Regulating TLR4/MyD88/NF-κB Pathway

被引:10
作者
Guo, Dengjun [1 ]
Zhou, Jie [2 ]
Zhang, Meng [3 ]
Taximaimaiti, Reyisha [4 ]
Wang, Xiaoping [4 ]
Wang, Hai [3 ]
机构
[1] Tongde Hosp Zhejiang Prov, Dept Neurol, Hangzhou 310012, Zhejiang, Peoples R China
[2] Zhejiang Acad Tradit Chinese Med, Ctr Med Resources Res, Hangzhou 310012, Zhejiang, Peoples R China
[3] Tongde Hosp Zhejiang Prov, Dept Clin Lab, Hangzhou 310012, Zhejiang, Peoples R China
[4] Shanghai Jiao Tong Univ, Tongren Hosp, Dept Neurol, Sch Med, Shanghai 200336, Peoples R China
关键词
AMELIORATE OXIDATIVE STRESS; L; NEUROINFLAMMATION; INFLAMMATION; ANTIOXIDANT; EXTRACTION; EXPRESSION; APOPTOSIS; TISSUE;
D O I
10.1155/2021/5575636
中图分类号
O63 [高分子化学(高聚物)];
学科分类号
070305 ; 080501 ; 081704 ;
摘要
Objective. To investigate the potential role of Momordica charantia polysaccharides (MCPs) in Parkinson's disease (PD) and reveal the molecular mechanism of its function. Method. 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) and 1-methyl-4-phenylpyridinium (1-methyl-4-phenylpyridinium, MPP+) were used to establish PD mice and cell models. The mice and cells were divided into 4 groups: Control group, Control+MCPs group, PD group, and PD+MCPs group. Pole climbing experiment and Rotarod experiment were used to observe the coordination ability of mice. High-performance liquid chromatography and enzyme-linked immunosorbent assay (ELISA) were used to determine neurotransmitters and metabolites, inflammatory factors TNF-alpha and IL-1 beta, oxidative stress-related markers SOD, MDA, and GSH in striatum tissues. Western blot was used to determine the protein levels of tyrosine hydroxylase (TH), oxidative stress-related protein Cytochrome C (Cytochrome C), and apoptosis-related proteins Bcl-2, Bax, and cleaved Caspase-3 in tissues and cells. Moreover, flow cytometry, PI staining, and fluorescence were used to observe cell apoptosis. Finally, the activation effect of MCPs on TLR4/MyD88/NF-kappa B signaling pathway was observed and verified. Results. Compared with the Control group, MPTP treatment can induce brain damage in mice (all P<0.05), change the metabolic state of neurotransmitters (all P<0.05), induce inflammation (all P<0.05), and induce apoptosis and the occurrence of oxidation reaction (all P<0.05); however, MCPs treatment can significantly reverse the above changes (all P<0.05). In cell models, studies have found that MCPs can play a protective role by regulating the activation state of TLR4/MyD88/NF-kappa B pathway. Conclusion. This study found that the application of MCPs therapy can play anti-inflammatory, antioxidative stress, and antiapoptotic effects in PD by regulating the activation of the TLR4/MyD88/NF-kappa B pathway.
引用
收藏
页数:15
相关论文
共 50 条
[1]  
Balestrino R, 2020, EUR J NEUROL, V27, P27
[2]  
Beitz Janice M, 2014, Front Biosci (Schol Ed), V6, P65
[3]   Tinospora cordifolia Suppresses Neuroinflammation in Parkinsonian Mouse Model [J].
Birla, Hareram ;
Rai, Sachchida Nand ;
Sen Singh, Saumitra ;
Zahra, Walia ;
Rawat, Arun ;
Tiwari, Neeraj ;
Singh, Rakesh K. ;
Pathak, Abhishek ;
Singh, Surya Pratap .
NEUROMOLECULAR MEDICINE, 2019, 21 (01) :42-53
[4]   NEW METHOD FOR QUANTITATIVE-DETERMINATION OF URONIC ACIDS [J].
BLUMENKR.N ;
ASBOEHAN.G .
ANALYTICAL BIOCHEMISTRY, 1973, 54 (02) :484-489
[5]   Neuroprotective effects of geniposide in the MPTP mouse model of Parkinson's disease [J].
Chen, YiMei ;
Zhang, Yanfang ;
Li, Lin ;
Hoelscher, Christian .
EUROPEAN JOURNAL OF PHARMACOLOGY, 2015, 768 :21-27
[6]   Parkinson disease: from pathology to molecular disease mechanisms [J].
Dexter, David T. ;
Jenner, Peter .
FREE RADICAL BIOLOGY AND MEDICINE, 2013, 62 :132-144
[7]   Protection of Momordica charantia polysaccharide against intracerebral hemorrhage-induced brain injury through JNK3 signaling pathway [J].
Duan, Zhen-Zhen ;
Zhou, Xiao-Ling ;
Li, Yi-Hang ;
Zhang, Feng ;
Li, Feng-Ying ;
Qi Su-Hua .
JOURNAL OF RECEPTORS AND SIGNAL TRANSDUCTION, 2015, 35 (06) :523-529
[8]   Gene-by-environment interactions in Alzheimer's disease and Parkinson's disease [J].
Dunn, Amy R. ;
O'Connell, Kristen M. S. ;
Kaczorowski, Catherine C. .
NEUROSCIENCE AND BIOBEHAVIORAL REVIEWS, 2019, 103 :73-80
[9]   Development of an enzyme-linked immunosorbent assay (ELISA) to measure the level of tyrosine hydroxylase protein in brain tissue from Parkinson's disease models [J].
Fauss, Donald ;
Motter, Ruth ;
Dofiles, Lilibeth ;
Rodrigues, Maria Armanda Viana ;
You, Monica ;
Diep, Linnea ;
Yang, Yangli ;
Seto, Pui ;
Tanaka, Kevin ;
Baker, Jeanne ;
Bergeron, Marcelle .
JOURNAL OF NEUROSCIENCE METHODS, 2013, 215 (02) :245-257
[10]  
Frasca L, 2018, FRONT IMMUNOL, V9, DOI [10.3389/fimmu.2018.01936, 10.3389/fimmu.2018.02363]