PLANTLET REGENERATION THROUGH SOMATIC EMBRYOGENESIS IN SCHISANDRA CHINENSIS (TURCZ.) BAILL. AND ANALYSIS OF GENETIC STABILITY OF REGENERATED PLANTS BY SRAP MARKERS

To study the efficient plant regeneration system of Schisandra chinensis (Turcz.) Baill. via somatic embryogenesis and evaluated the genetic stability of regenerated plants was carried out. Dormant buds were cultured on Murashige and 'Skoog (MS) medium supplemented with 2,4-D and TDZ for induction of callus. The callus induction rate was highest on MS medium with 3.0 mg/l 2,4-D and 0.2 mg/l TDZ and MS medium supplemented with 1.0 mg/l TDZ and 0.2 mg/l zeatin (Zt) was beneficial to embryonic callus induction. The globular embryo formation was improved when embryonic calli were cultured in liquid half-strength MS medium than semi-solid medium. MS supplemented with 0.2 mg/l indole-3-butytric acid (IBA) was most favorable for root formation of regenerated plants. SRAP primer were used for PCR amplification on parental and regenerated plants, and there were no specific bands produced, confirming that the plant regeneration system was stable and reliable. The result of this study will be beneficial for genetic transformation and mass clonal propagation.