Quantification method for elemental bio-imaging by LA-ICP-MS using metal spiked PMMA films

被引：71
作者：
Austin, Christine [1 ]
Hare, Dominic [1 ]
Rawling, Tristan [1 ]
McDonagh, Andrew M. [1 ]
Doble, Philip [1 ]
机构：
[1] Univ Technol Sydney, Elemental Bioimaging Facil, Dept Chem & Forens Sci, Sydney, NSW 2007, Australia
来源：
JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY | 2010年 / 25卷 / 05期
关键词：
INDUCTIVELY-COUPLED PLASMA; LASER-ABLATION; MASS-SPECTROMETRY; THIN-SECTIONS; TRACE-ELEMENTS; BIOLOGICAL TISSUES; HUMAN BRAIN; COPPER; ZINC; NORMALIZATION;
D O I：
10.1039/b911316a
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A method for quantitative analysis of biological soft tissues by laser ablation-inductively coupled plasma-mass spectrometry has been developed. Polymer film standards were produced by spin coating spiked solutions of polymethylmethacrylate onto quartz substrates. Calibration curves throughout the range of 0-400 mu g g(-1) yielded correlation coefficients better than 0.999 for Zn-66 and Cu-63. Spiked, homogenised soft tissue standards were quantified by LA-ICP-MS against the thin film standards. The results agreed with values calculated from solution nebulisation ICP-MS. A procedure for internal standardisation by employing ruthenium or yttrium in the underlying thin film was also assessed.
引用
收藏
页码：722 / 725
页数：4
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