Liver organoid as a 3D in vitro model for drug validation and toxicity assessment

被引：76

作者：

Brooks, Anastasia ^{[1
,2
]}

Liang, Xiaowen ^{[1
,3
]}

Zhang, Yonglong ^{[4
]}

Zhao, Chun-Xia ^{[2
]}

Roberts, Michael S. ^{[1
,5
]}

Wang, Haolu ^{[1
,3
]}

Zhang, Lei ^{[4
]}

Crawford, Darrell H. G. ^{[3
,6
]}

机构：

[1] Univ Queensland, Univ Queensland Diamantina Inst, Brisbane, Qld, Australia

[2] Univ Queensland, Australian Inst Bioengn & Nanotechnol, St Lucia, Qld, Australia

[3] Greenslopes Private Hosp, Gallipoli Med Res Inst, Brisbane, Qld, Australia

[4] Univ Chinese Acad Sci, Chinese Acad Sci, Shanghai Inst Biochem & Cell Biol, State Key Lab Cell Biol,CAS Ctr Excellence Mol Ce, Shanghai, Peoples R China

[5] Univ South Australia, Sch Pharm & Med Sci, Adelaide, SA, Australia

[6] Univ Queensland, Fac Med, Sch Clin Med, Brisbane, Qld, Australia

来源：

PHARMACOLOGICAL RESEARCH | 2021年 / 169卷

基金：

英国医学研究理事会;

关键词：

Liver organoid; Drug validation; Toxicity assessment; PLURIPOTENT STEM-CELLS; HEPATIC STELLATE CELLS; DISEASE; CULTURE; CHOLANGIOCYTES; HOMEOSTASIS; GENERATION; FIBROSIS; INJURY; MOUSE;

D O I：

10.1016/j.phrs.2021.105608

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

The past decade has seen many advancements in the development of three-dimensional (3D) in vitro models in pharmaceutical sciences and industry. Specifically, organoids present a self-organising, self-renewing and more physiologically relevant model than conventional two-dimensional (2D) cell cultures. Liver organoids have been developed from a variety of cell sources, including stem cells, cell lines and primary cells. They have potential for modelling patient-specific disease and establishing personalised therapeutic approaches. Additionally, liver organoids have been used to test drug efficacy and toxicity. Herein we summarise cell sources for generating liver organoids, the advantages and limitations of each cell type, as well as the application of the organoids in modelling liver diseases. We focus on the use of liver organoids as tools for drug validation and toxicity assessment.

引用

页数：8

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