DETERMINATION OF HYDROXYPROLINE USING ION-EXCHANGE LIQUID CHROMATOGRAPHY WITH VIS DETECTOR AND HIGH PERFORMANCE LIQUID CHROMATOGRAPHY WITH FLUORESCENCE DETECTOR

被引：0

作者：

Lackova, Zuzana ^{[1
,3
]}

Cernei, Natalia ^{[3
]}

Sterbova, Dagmar ^{[1
]}

Haddad, Yazan ^{[1
,3
]}

Rozikova, Veronika ^{[2
]}

Komprda, Tomas ^{[2
]}

Zitka, Ondrej ^{[1
,3
]}

机构：

[1] Mendel Univ Brno, Dept Chem & Biochem, Zemedelska 1, Brno 61300, Czech Republic

[2] Mendel Univ Brno, Dept Food Technol, Zemedelska 1, Brno 61300, Czech Republic

[3] Brno Univ Technol, Cent European Inst Technol, Tech 10, Brno 61600, Czech Republic

来源：

PROCEEDINGS OF 24TH INTERNATIONAL PHD STUDENTS CONFERENCE (MENDELNET 2017) | 2017年

关键词：

hydroxyproline; HPLC-FLD; IEC-VIS; microwave hydrolysis; pig skin; CORNEAL FIBROBLASTS; PULMONARY-FIBROSIS; COLLAGEN; RATS;

D O I：

暂无

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

The first aim of the experiment was an optimization of the method for determination of hydroxyproline in a pig skin using high-performance liquid chromatography with fluorescence detector (HPLC-FLD) and ion-exchange liquid chromatography with VIS detector (IEC-VIS). On the basis of the experiments performed, it was found that HPLC-FLD method is three times more sensitive than IEC-VIS method. For IEC analysis of hydroxyproline, the limit of detection (LOD) was 4.10 mu g/ml whereas the limit of quantification (LOQ) was 13.50 mu g/ml. For HPLC analysis of hydroxyproline, the limit of detection (LOD) was 1 ng/ml whereas the limit of quantification (LOQ) was 3 ng/ml. The second aim was the testing of influence of different volume of 6M HCl on extraction of sample (50 mg) for analysis using HPLC-FLD. Here it was found that the best volume was 250 I 6M HCl.

引用

页码：905 / 910

页数：6

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