Three-dimensional resolution and contrast-enhanced confocal microscopy with array detection

被引：20

作者：

Ge, Baoliang ^{[1
]}

Wang, Yifan ^{[1
]}

Huang, Yujia ^{[1
]}

Kuang, Cuifang ^{[1
]}

Fang, Yue ^{[1
]}

Xiu, Peng ^{[1
]}

Rong, Zihao ^{[1
]}

Liu, Xu ^{[1
]}

机构：

[1] Zhejiang Univ, Coll Opt Sci & Engn, State Key Lab Modern Opt Instrumentat, Hangzhou 310027, Zhejiang, Peoples R China

来源：

OPTICS LETTERS | 2016年 / 41卷 / 09期

基金：

中国国家自然科学基金;

关键词：

FLUORESCENCE; SUPERRESOLUTION;

D O I：

10.1364/OL.41.002013

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

What we believe is a novel method for improving confocal microscopy's resolution and contrast in 3D space is proposed. Based on a conventional confocal microscopy setup, we use an array detector composed of 32 photomultiplier tubes (PMTs) to replace one point-detector, where the location offset of each PMT caused a different effective point spread function (PSF). By applying array detection and the fluorescence emission difference method of an image with a solid PSF and another with a donut-shaped PSF, we can enhance lateral resolution about 27% in real time with only one scan, and improve the axial resolving ability by about 22% simultaneously. Experimental results of both fluorescent beads and living cells are presented to verify the applicability and effectiveness of our method. (C) 2016 Optical Society of America

引用

页码：2013 / 2016

页数：4

共 18 条

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