The Effect of Protease Inhibitors on Digestive Proteolytic Activity in the Raspberry Weevil, Aegorhinus superciliosus (Gu,rin) (Coleoptera: Curculionidae)

被引：8

作者：

Medel, V. ^{[1
,2
]}

Palma, R. ^{[3
]}

Mercado, D. ^{[1
]}

Rebolledo, R. ^{[2
]}

Quiroz, A. ^{[1
]}

Mutis, A. ^{[1
]}

机构：

[1] Univ La Frontera, Lab Quim Ecol, Dept Ciencias Quim & Recursos Nat, Temuco, Araucania, Chile

[2] Univ La Frontera, Entomol Lab, Dept Ciencias Agron & Recursos Nat, Temuco, Araucania, Chile

[3] Univ Talca, Inst Ciencias Biol, Lab Interacc Insectoplanta, Talca, Chile

来源：

NEOTROPICAL ENTOMOLOGY | 2015年 / 44卷 / 01期

关键词：

Enzymatic characterization; insecticidal effect; trypsin-like serine protease; LARVAL HELIOTHIS-ZEA; PROTEINASE-INHIBITORS; HUBNER LEPIDOPTERA; CATHEPSIN-D; MIDGUT; TRYPSIN; PURIFICATION; ENZYMES; GROWTH; IDENTIFICATION;

D O I：

10.1007/s13744-014-0250-9

中图分类号：

Q96 [昆虫学];

学科分类号：

摘要：

The raspberry weevil, Aegorhinus superciliosus (Gu,rin) (Coleoptera: Curculionidae), is an economically important pest of blueberry in southern Chile. The digestive protease activity of adult insects was investigated using general and specific substrates and inhibitors. Enzymatic assays demonstrated the presence of trypsin- and chymotrypsin-like serine proteinases. Furthermore, in vitro assays using phenylmethylsulfonyl fluoride (PMSF) and soybean trypsin inhibitor (SBTI) at 0.01 and 0.1 mM showed percentages of enzymatic inhibition between 0 and 16% for PMSF and 67 to 76% for SBTI, whereas in vivo assays indicated that SBTI caused between 50 and 90% mortality in males and between 80 and 100% in females. Our data indicate the presence of serine proteases and suggest that digestive proteases could be a target for the design and development of strategies to control the raspberry weevil.

引用

页码：77 / 83

页数：7

共 49 条

[1]

Aguilera A, 1995, SEM PROT VEG INIA CA

[2]

Aguilera P. Alfonso, 2001, Revista Chilena de Entomologia, V28, P5

[3] Purification and characterization of a digestive cathepsin D proteinase isolated from Tribolium castaneum larvae (Herbst) [J].

BlancoLabra, A ;

MartinezGallardo, NA ;

SandovalCardoso, L ;

DelanoFrier, J .

INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY, 1996, 26 (01) :95-100

[4]

BONETE MJ, 1984, COMP BIOCHEM PHYS B, V78, P207, DOI 10.1016/0305-0491(84)90170-6

[5] PLANT PROTEINASE-INHIBITORS - MECHANISM OF ACTION AND EFFECT ON THE GROWTH AND DIGESTIVE PHYSIOLOGY OF LARVAL HELIOTHIS-ZEA AND SPODOPTERA-EXIQUA [J].

BROADWAY, RM ;

DUFFEY, SS .

JOURNAL OF INSECT PHYSIOLOGY, 1986, 32 (10) :827-833

[6] THE EFFECT OF DIETARY-PROTEIN ON THE GROWTH AND DIGESTIVE PHYSIOLOGY OF LARVAL HELIOTHIS-ZEA AND SPODOPTERA-EXIGUA [J].

BROADWAY, RM ;

DUFFEY, SS .

JOURNAL OF INSECT PHYSIOLOGY, 1986, 32 (08) :673-680

[7] PARTIAL-PURIFICATION AND CHARACTERIZATION OF THE MAJOR MIDGUT PROTEASES OF GRASS GRUB LARVAE (COSTELYTRA-ZEALANDICA, COLEOPTERA, SCARABAEIDAE) [J].

CHRISTELLER, JT ;

SHAW, BD ;

GARDINER, SE ;

DYMOCK, J .

INSECT BIOCHEMISTRY, 1989, 19 (03) :221-+

[8]

Dorrah Moataza A., 2004, Efflatounia, V4, P23

[9] A chymotrypsin-like proteinase from the midgut of Tenebrio molitor larvae [J].

Elpidina, EN ;

Tsybina, TA ;

Dunaevsky, YE ;

Belozersky, MA ;

Zhuzhikov, DP ;

Oppert, B .

BIOCHIMIE, 2005, 87 (08) :771-779

[10]

France I. A., 2000, Agricultura Tecnica, V60, P205

← 1 2 3 4 5 →