Independent Evaluation of Cell Culture Systems for Hepatitis E Virus

被引:12
作者
Chew, Nicholas [1 ]
Situ, Jianwen [1 ]
Wu, Shusheng [1 ]
Yao, Weiming [1 ]
Sridhar, Siddharth [1 ,2 ,3 ]
机构
[1] Univ Hong Kong, Li Ka Shing Fac Med, Sch Clin Med, Dept Microbiol, Hong Kong, Peoples R China
[2] Univ Hong Kong, State Key Lab Emerging Infect Dis, Hong Kong, Peoples R China
[3] Univ Hong Kong, Carol Yu Ctr Infect, Hong Kong, Peoples R China
来源
VIRUSES-BASEL | 2022年 / 14卷 / 06期
关键词
hepatitis E virus; cell culture; wild-type HEV growth; rat HEV; INFECTION; MEMBRANE; REPLICATION; HEV;
D O I
10.3390/v14061254
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Hepatitis E virus (HEV) infection in humans is primarily caused by genotypes within Paslahepevirus species balayani (HEV-A). Rocahepevirus species ratti (HEV-C1, otherwise known as rat HEV) can also infect humans. HEV grows poorly in cell culture. Recent studies have reported that hyper-confluent cell layers, amphotericin B, MgCl2, progesterone, and dimethyl sulfoxide (DMSO) increase HEV yield in vitro. Here, we describe an independent evaluation of the effectiveness of these modifications in improving the yield of HEV-A genotype 4 (HEV-A4) and HEV-C1 from clinical samples in PLC/PRF/5 cells. We found that amphotericin B, MgCl2, and DMSO increased HEV yield from high-viral-load patient stool samples, while progesterone was not effective. Yield of HEV-C1 was lower than HEV-A4 across all medium conditions, but was boosted by DMSO. HEV-A4 could be maintained for over 18 months in amphotericin B- and MgCl2-containing medium, with the demonstration of viral antigen in supernatants and infected cells. We also evaluated various protocols to remove pseudo-envelopes from cell culture-derived HEV. Treating cell culture supernatant with NP-40 was the most effective. Our findings identify key modifications that boost HEV growth in vitro and illustrate the importance of independent verification of such studies using diverse HEV variants and cell lines.
引用
收藏
页数:12
相关论文
共 37 条
[1]   Rat Hepatitis E Virus Linked to Severe Acute Hepatitis in an Immunocompetent Patient [J].
Andonov, Anton ;
Robbins, Mark ;
Borlang, Jamie ;
Cao, Jingxin ;
Hatchette, Todd ;
Stueck, Ashley ;
Deschambault, Yvon ;
Murnaghan, Kyle ;
Varga, Jessy ;
Johnston, Lynn .
JOURNAL OF INFECTIOUS DISEASES, 2019, 220 (06) :951-955
[2]   Replication of hepatitis E virus in three-dimensional cell culture [J].
Berto, A. ;
Van der Poel, W. H. M. ;
Hakze-van der Honing, R. ;
Martelli, F. ;
La Ragione, R. M. ;
Inglese, N. ;
Collins, J. ;
Grierson, S. ;
Johne, R. ;
Reetz, J. ;
Dastjerdi, A. ;
Banks, M. .
JOURNAL OF VIROLOGICAL METHODS, 2013, 187 (02) :327-332
[3]   Molecular biology and replication of hepatitis E virus [J].
Cao, Dianjun ;
Meng, Xiang-Jin .
EMERGING MICROBES & INFECTIONS, 2012, 1
[4]   Optimized Hepatitis E Virus (HEV) Culture and Its Application to Measurements of HEV Infectivity [J].
Capelli, Nicolas ;
Dubois, Martine ;
Pucelle, Melanie ;
Da Silva, Isabelle ;
Lhomme, Sebastien ;
Abravanel, Florence ;
Chapuy-Regaud, Sabine ;
Izopet, Jacques .
VIRUSES-BASEL, 2020, 12 (02)
[5]   Hepatitis E Virus Enters Liver Cells Through a Dynamin-2, Clathrin and Membrane Cholesterol-Dependent Pathway [J].
Holla, Prasida ;
Ahmad, Imran ;
Ahmed, Zulfazal ;
Jameel, Shahid .
TRAFFIC, 2015, 16 (04) :398-416
[6]   Cell culture of sporadic hepatitis E virus in China [J].
Huang, RT ;
Li, DR ;
Wei, SJ ;
Li, QH ;
Yuan, XT ;
Geng, LQ ;
Li, XY ;
Liu, MX .
CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY, 1999, 6 (05) :729-733
[7]   Phenotypic characterization of cell culture-derived hepatitis E virus subjected to different chemical treatments: Application in virus removal via nanofiltration [J].
Ideno, Shoji ;
Inoue, Takamasa ;
Takahashi, Kadue ;
Urayama, Takeru ;
Maeno, Hideki ;
Takeuchi, Kaoru ;
Sakai, Kaoru .
JOURNAL OF VIROLOGICAL METHODS, 2021, 296
[8]   Rat hepatitis E virus derived from wild rats (Rattus rattus) propagates efficiently in human hepatoma cell lines [J].
Jirintai, Suljid ;
Tanggis ;
Mulyanto ;
Suparyatmo, Joseph Benedictus ;
Takahashi, Masaharu ;
Kobayashi, Tominari ;
Nagashima, Shigeo ;
Nishizawa, Tsutomu ;
Okamoto, Hiroaki .
VIRUS RESEARCH, 2014, 185 :92-102
[9]   Detection of a novel hepatitis E-like virus in faeces of wild rats using a nested broad-spectrum RT-PCR [J].
Johne, Reimar ;
Plenge-Boenig, Anita ;
Hess, Michael ;
Ulrich, Rainer G. ;
Reetz, Jochen ;
Schielke, Anika .
JOURNAL OF GENERAL VIROLOGY, 2010, 91 :750-758
[10]   Characteristics of Autochthonous Hepatitis E Virus Infection in Solid-Organ Transplant Recipients in France [J].
Legrand-Abravanel, Florence ;
Kamar, Nassim ;
Sandres-Saune, Karine ;
Garrouste, Cyril ;
Dubois, Martine ;
Mansuy, Jean-Michel ;
Muscari, Fabrice ;
Sallusto, Federico ;
Rostaing, Lionel ;
Izopet, Jacques .
JOURNAL OF INFECTIOUS DISEASES, 2010, 202 (06) :835-844