Rapid and direct method for extraction of RNA from soil

被引：25
作者：
Borneman, J
Triplett, EW
机构：
[1] Univ Wisconsin, Dept Agron, Brock Inst Environm Microbiol, Madison, WI 53706 USA
[2] Univ Wisconsin, Dept Soil Sci, Madison, WI 53706 USA
来源：
SOIL BIOLOGY & BIOCHEMISTRY | 1997年 / 29卷 / 11-12期
关键词：
D O I：
10.1016/S0038-0717(97)00084-9
中图分类号：
S15 [土壤学];
学科分类号：
0903 ; 090301 ;
摘要：
A rapid method for direct extraction of RNA from soil was developed. It is based on cell lysis by a bead beater procedure that optimizes extraction efficiency and minimizes shearing of RNA. The RNA was extracted from three very different soils and was free of DNA contamination. The extracted RNA was sufficiently pure to permit amplification by RT-PCR. Intact rRNA molecules of both prokaryotic and eukaryotic origin were extracted by this method and were of sufficient yield to visualize these molecules on agarose gels. (C) 1997 Elsevier Science Ltd.
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页码：1621 / 1624
页数：4
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