Molecular pathology analyses of two fatal human infections of avian influenza A(H7N9) virus

被引：20

作者：

Feng, Yanling ^{[1
]}

Hu, Lvyin ^{[2
]}

Lu, Shuihua ^{[3
]}

Chen, Qingguo ^{[3
]}

Zheng, Ye ^{[1
]}

Zeng, Dong ^{[1
]}

Zhang, Jun ^{[2
]}

Zhang, Anli ^{[4
]}

Chen, Liang ^{[5
]}

Hu, Yunwen ^{[6
]}

Zhang, Zhiyong ^{[7
]}

机构：

[1] Fudan Univ, Dept Pathol, Shanghai Publ Hlth Clin Ctr, Shanghai 200433, Peoples R China

[2] Fudan Univ, Dept Clin Lab, Shanghai Publ Hlth Clin Ctr, Shanghai 200433, Peoples R China

[3] Fudan Univ, Dept Resp Med, Shanghai Publ Hlth Clin Ctr, Shanghai 200433, Peoples R China

[4] Fudan Univ, Sci Res Ctr, Shanghai Publ Hlth Clin Ctr, Shanghai 200433, Peoples R China

[5] Fudan Univ, Dept Viral Hepatitis, Shanghai Publ Hlth Clin Ctr, Shanghai 200433, Peoples R China

[6] Fudan Univ, Dept Pathogen Diag & Biosafety, Shanghai Publ Hlth Clin Ctr, Shanghai 200433, Peoples R China

[7] Fudan Univ, Dept Radiol, Shanghai Publ Hlth Clin Ctr, Shanghai 200433, Peoples R China

来源：

JOURNAL OF CLINICAL PATHOLOGY | 2015年 / 68卷 / 01期

基金：

中国国家自然科学基金;

关键词：

A H7N9 VIRUS; SURFACTANT PROTEIN-A; COMPLEMENT;

D O I：

10.1136/jclinpath-2014-202441

中图分类号：

R36 [病理学];

学科分类号：

100104 ;

摘要：

Aims To investigate the histopathological manifestations of two fatal cases of H7N9 influenza A virus infection. Methods Pulmonary and hepatic specimens from two fatal cases of H7N9 influenza virus infection were examined using H&E staining. Additionally, in situ hybridisation was performed with probes (ViewRNA) targeting H7N9 RNA and IP-10, interleukin (IL)-6 mRNA. The distribution of surfactant protein A (SP-A), surfactant protein B (SP-B), CD3, CD4, CD8, CD68 and C4d were determined with immunohistochemistry. Results Apart from the typical diffuse alveolar damage and hyaline membrane observed in severe influenza infection, we detected H7N9 RNA and massive intrapulmonary production of IP-10 and IL-6 mRNA using in situ hybridisation. Hyperplasia of type II pneumocytes was observed by H&E staining and immunohistochemistry. Proliferating macrophages and clustered neutrophils in the infected lungs were observed, whereas T lymphocytes, especially CD4T helper cells, were markedly depleted. No obvious complement deposition was found in lung tissues. Conclusions Our findings suggest that H7N9 influenza virus induced an immunological response towards overt pulmonary inflammation and systemic lymphopenia which led to intense alveolar damage and respiratory failure.

引用

页码：57 / 63

页数：7

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