A coumarin-based fluorescent probe for specific detection of cysteine in the lysosome of living cells

被引：31

作者：

Lu, Guowei ^{[1
]}

Dong, Jianning ^{[1
]}

Fan, Congbin ^{[1
]}

Tu, Yayi ^{[1
]}

Pu, Shouzhi ^{[1
]}

机构：

[1] Jiangxi Sci & Technol Normal Univ, Jiangxi Key Lab Organ Chem, Nanchang 330013, Jiangxi, Peoples R China

来源：

BIOORGANIC CHEMISTRY | 2022年 / 119卷

基金：

中国国家自然科学基金;

关键词：

Cysteine; Acrylate; Coumarin; Fluorescent probe; Cell imaging; Lysosome-targetable; SELECTIVE FLUORESCENT; TOTAL HOMOCYSTEINE; GSH; GLUTATHIONE; PLASMA; CYS; ELECTROPHORESIS; PARKINSONS; SULFATE; THIOLS;

D O I：

10.1016/j.bioorg.2021.105558

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Cysteine (Cys), the only amino acid in the 20 natural amino acids that contains a reduced sulfhydryl group, plays important roles in the balance of redox homeostasis in biological systems. Lysosome is an important organelle containing a variety of hydrolases and has been proved to be the decomposition center of a variety of exogenous and endogenous macromolecular substances. In this research, a coumarin-based fluorescent probe MCA for the detection of Cys in lysosomes of living cells was developed. Due to the acrylate moiety, this probe exhibited high sensitivity (detection limit = 6.8 nM) and selectivity towards Cys superior to other analytes. Moreover, the probe was proved to be lysosome-targetable and showed good cell imaging ability and low cell toxicity.

引用

页数：7

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