Genetic relationship of Sinorhizobium meliloti and Sinorhizobium medicae strains isolated from Caucasian region

被引:54
作者
Biondi, EG
Pilli, E
Giuntini, E
Roumiantseva, ML
Andronov, EE
Onichtchouk, OP
Kurchak, ON
Simarov, BV
Dzyubenko, NI
Mengoni, A
Bazzicalupo, M [1 ]
机构
[1] Univ Florence, Dept Anim Biol & Genet, I-50125 Florence, Italy
[2] RIAM, St Petersburg 196608, Russia
[3] NI Vavilov Inst Plant Genet Resources, St Petersburg, Russia
关键词
Sinorhizobium meliloti; Sinorhizobium medicae; nodD fingerprinting; amplification fragment length polymorphism; intergenic gene spacer;
D O I
10.1016/S0378-1097(03)00098-3
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Sinorhizobium meliloti and Sinorhizobium medicae are two closely related species of the genus Sinorhizobiurn showing a similar host range, nodulating leguminous species of the genera Medicago, Melilotus and Trigonella, but their phylogenic relationship has not been elucidated yet. In this paper we report the application of three different molecular markers, (i) RFLP of nodD genes, (ii) 16S-23S rDNA intergenic gene spacer fingerprinting and (iii) amplification fragment length polymorphism to S. meliloti and S. medicae strains isolated from the Caucasian area, which is the region of origin of the host plant Medicago. The analysis of data could suggest the origin of S. medicae strains from an ancestral S. meliloti population. (C) 2003 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
引用
收藏
页码:207 / 213
页数:7
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