Analysis of lipoprotein-specific lipids in patients with acute coronary syndrome by asymmetrical flow field-flow fractionation and nanoflow liquid chromatography-tandem mass spectrometry

被引：21
作者：
Lee, Jae Hyun [1 ]
Yang, Joon Seon [1 ]
Lee, Sang-Hak [2 ,3 ]
Moon, Myeong Hee [1 ]
机构：
[1] Yonsei Univ, Dept Chem, Seoul 03722, South Korea
[2] Yonsei Univ, Coll Med, Severance Hosp, Dept Internal Med,Div Cardiol, Seoul 03722, South Korea
[3] Yonsei Univ, Coll Med, Cardiovasc Res Inst, Seoul 03722, South Korea
来源：
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2018年 / 1099卷
基金：
新加坡国家研究基金会;
关键词：
Acute coronary syndrome; Coronary artery disease; Lipidomie analysis; Lipoprotein; nUPLC-ESI-MS/MS; Flow field-flow fractionation; LOW-DENSITY-LIPOPROTEIN; ARTERY-DISEASE; CARDIOVASCULAR-DISEASE; RISK-FACTORS; LYSOPHOSPHATIDYLCHOLINE; ATHEROSCLEROSIS; PHOSPHOLIPIDS; PREDICTION; LIPIDOMICS; PROTEOMICS;
D O I：
10.1016/j.jchromb.2018.09.016
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A comprehensive lipid analysis was performed at the plasma lipoprotein level in patients with acute coronary syndrome (ACS) and stable coronary artery disease (CAD). Because the lipids in lipoproteins are related to the pathology of the cardiovascular system, lipoprotein-specific lipid analysis can be useful for understanding the mechanism of lipid-associated cardiovascular diseases. Lipoproteins were size-sorted into high density lipoproteins (HDL) and low density lipoproteins (LDL) using asymmetrical flow field-flow fractionation, then lipids of each lipoprotein were analysed using nanoflow ultrahigh performance liquid chromatography-electrospray ionization-tandem mass spectrometry. A total of 365 lipids were structurally identified and quantified by selected reaction monitoring method. Two high abundance lysophosphatidylcholines (16:0 and 18:0) were significantly increased only in the HDL of the ACS group (vs. the stable CAD group). Phosphatidylethanolamines (38:5 and 40:5) significantly increased in ACS by > 2-fold in both lipoproteins. (18:0, 22:6)-diacylglycerol increased in ACS by 3.5-fold only in LDL; however, most high abundance triacylglycerols decreased 2-fold in both lipoproteins. The present study revealed the usefulness of lipoprotein-specific analysis of lipids in distinguishing ACS from stable CAD, and the selected lipids analysed in this study may be useful in the development of lipid markers for the early detection of ACS.
引用
收藏
页码：56 / 63
页数：8
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