Distance Determination in Proteins inside Xenopus laevis Oocytes by Double Electron-Electron Resonance Experiments

被引：99
作者：
Igarashi, Ryuji [1 ,2 ]
Sakai, Tomomi [3 ]
Hara, Hideyuki [4 ]
Tenno, Takeshi [2 ,5 ]
Tanaka, Toshiaki [6 ]
Tochio, Hidehito [1 ,2 ]
Shirakawa, Masahiro [1 ,2 ,7 ]
机构：
[1] Kyoto Univ, Grad Sch Engn, Kyoto 6158510, Japan
[2] JST, CREST, Kawaguchi, Saitama 3320012, Japan
[3] Mitsubishi Kagaku Inst Life Sci, Tokyo 1948511, Japan
[4] Bruker Biospin KK, Kanagawa 2210022, Japan
[5] Kobe Univ, Grad Sch Med, Kobe, Hyogo 6500017, Japan
[6] Tokyo Inst Technol, Fac Biosci & Biotechnol, Kanagawa 2268501, Japan
[7] Yokohama Inst, RIKEN, Kanagawa 2300045, Japan
来源：
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2010年 / 132卷 / 24期
基金：
日本科学技术振兴机构;
关键词：
CELL NMR-SPECTROSCOPY; ACTIVATION; ESR;
D O I：
10.1021/ja906104e
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
DEER (double electron-electron resonance) enables the observation of long-range dipole interactions (1.5-8 nm) between electron spin centers and has become a unique method for structural analysis of site-directed spin-labeled (SDSL) proteins. The method was applied to proteins inside eukaryotic cells, Xenopus laevis oocytes. DEER measurements of the oocytes, into which SDSL-ubiquitin derivates were injected, gave rise to interpretable signals and allowed us to perform in situ analyses of the interspin distances of the proteins.
引用
收藏
页码：8228 / +
页数：4
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