Effects of short and long incubations on DNA fragmentation of testicular sperm

被引:68
作者
Dalzell, LH
McVicar, CM
McClure, N
Lutton, D
Lewis, SEM
机构
[1] Queens Univ Belfast, Sch Med, Inst Clin Sci, Belfast BT12 6BJ, Antrim, North Ireland
[2] Reg Fertil Ctr, Royal Jubilee Matern Serv, Belfast, Antrim, North Ireland
关键词
D O I
10.1016/j.fertnstert.2004.04.053
中图分类号
R71 [妇产科学];
学科分类号
100211 ;
摘要
DNA fragmentation in testicular sperm from men with obstructive azoospermia is increased by 4-hour and 24-hour incubations and after cryopreservation with the effect is intensified by post-thaw incubation. Testicular sperm for use in intracytoplasmic sperm injection (ICSI) should be injected without delay.(C) 2004 by American Society for Reproductive Medicine.
引用
收藏
页码:1443 / 1445
页数:3
相关论文
共 29 条
[1]  
Aitken RJ, 1997, MOL REPROD DEV, V47, P468, DOI 10.1002/(SICI)1098-2795(199708)47:4<468::AID-MRD14>3.0.CO
[2]  
2-S
[3]   Relative impact of oxidative stress on the functional competence and genomic integrity of human spermatozoa [J].
Aitken, RJ ;
Gordon, E ;
Harkiss, D ;
Twigg, JP ;
Milne, P ;
Jennings, Z ;
Irvine, DS .
BIOLOGY OF REPRODUCTION, 1998, 59 (05) :1037-1046
[4]   Free radicals, lipid peroxidation and sperm function [J].
Aitken, RJ .
REPRODUCTION FERTILITY AND DEVELOPMENT, 1995, 7 (04) :659-668
[5]   In-vitro culture of spermatozoa induces motility and increases implantation and pregnancy rates after testicular sperm extraction and intracytoplasmic sperm injection [J].
Balaban, B ;
Urman, B ;
Sertac, A ;
Alatas, C ;
Aksoy, S ;
Mercan, R ;
Nuhoglu, A .
HUMAN REPRODUCTION, 1999, 14 (11) :2808-2811
[6]  
BIGGERS J. D., 1971, Methods in mammalian embryology, P86
[7]   Effects of long-term in vitro incubation of human spermatozoa:: functional parameters and catalase effect [J].
Calamera, JC ;
Fernandez, PJ ;
Buffone, MG ;
Acosta, AA ;
Doncel, GF .
ANDROLOGIA, 2001, 33 (02) :79-86
[8]   Production of reactive oxygen species by spermatozoa undergoing cooling, freezing, and thawing [J].
Chatterjee, S ;
Gagnon, C .
MOLECULAR REPRODUCTION AND DEVELOPMENT, 2001, 59 (04) :451-458
[9]   Effects of 24-hour incubation after freeze-thawing on DNA fragmentation of testicular sperm from infertile and fertile men [J].
Dalzell, LH ;
Thompson-Cree, MEM ;
McClure, N ;
Traub, AI ;
Lewis, SEM .
FERTILITY AND STERILITY, 2003, 79 :1670-1672
[10]   PREGNANCIES AFTER TESTICULAR SPERM EXTRACTION AND INTRACYTOPLASMIC SPERM INJECTION IN NONOBSTRUCTIVE AZOOSPERMIA [J].
DEVROEY, P ;
LIU, J ;
NAGY, Z ;
GOOSSENS, A ;
TOURNAYE, H ;
CAMUS, M ;
VANSTEIRTEGHEM, A ;
SILBER, S .
HUMAN REPRODUCTION, 1995, 10 (06) :1457-1460