Differentiation of smoked Salmo salar, Oncorhynchus mykiss and Brama raii using the nuclear marker 5S rDNA

被引：38

作者：

Carrera, E ^{[1
]}

García, T ^{[1
]}

Céspedes, A ^{[1
]}

González, I ^{[1
]}

Fernández, A ^{[1
]}

Asensio, LM ^{[1
]}

Hernández, PE ^{[1
]}

Martín, R ^{[1
]}

机构：

[1] Univ Complutense Madrid, Fac Vet, Dept Nutr & Bromatol 3, E-28040 Madrid, Spain

来源：

INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY | 2000年 / 35卷 / 04期

关键词：

PCR; smoked fish; species identification;

D O I：

10.1046/j.1365-2621.2000.00404.x

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

PCR amplification of 5S rDNA was applied to differentiate smoked samples of Atlantic salmon (Salmo salar), rainbow trout (Oncorhynchus mykiss) and bream (Brama raii). Contiguous divergent primers for the conserved coding region of the 5S rRNA gene allowed species-specific amplification of tandemly arranged units of the 5S rDNA in these three related species. Thus, the 5S rDNA genetic marker may become very useful for inspection programmes intended to assess the species identity of smoked products.

引用

页码：401 / 406

页数：6

共 39 条

[1] THE USE OF HPLC PROTEIN PROFILES IN FISH SPECIES IDENTIFICATION [J].

ARMSTRONG, SG ;

LEACH, DN ;

WYLLIE, SG .

FOOD CHEMISTRY, 1992, 44 (02) :147-155

[2] NUCLEOTIDE-SEQUENCE OF THE 5S RNA GENE AND FLANKING REGIONS INTERSPERSED WITH HISTONE GENES IN ARTEMIA [J].

BAGSHAW, JC ;

SKINNER, HB ;

BURN, TC ;

PERRY, BA .

NUCLEIC ACIDS RESEARCH, 1987, 15 (08) :3628-3628

[3]

BARTLETT SE, 1992, BIOTECHNIQUES, V12, P408

[4] MOLECULAR ARRANGEMENT AND EVOLUTION OF HETEROCHROMATIC DNA [J].

BRUTLAG, DL .

ANNUAL REVIEW OF GENETICS, 1980, 14 :121-144

[5] Development of an enzyme-linked immunosorbent assay for the identification of smoked salmon (Salmo salar), trout (Oncorhynchus mykiss) and bream (Brama raii) [J].

Carrera, E ;

Martin, R ;

Garcia, T ;

Gonzalez, I ;

Sanz, B ;

Hernandez, PE .

JOURNAL OF FOOD PROTECTION, 1996, 59 (05) :521-524

[6]

Carrera E, 1997, J SCI FOOD AGR, V74, P547, DOI 10.1002/(SICI)1097-0010(199708)74:4<547::AID-JSFA828>3.0.CO

[7]

2-Q

[8] Identification of Atlantic salmon (Salmo salar) and rainbow trout (Oncorhynchus mykiss) by using polymerase chain reaction amplification and restriction analysis of the mitochondrial cytochrome b gene [J].

Carrera, E ;

Garcia, T ;

Cespedes, A ;

Gonzalez, I ;

Sanz, B ;

Hernandez, PE ;

Martin, R .

JOURNAL OF FOOD PROTECTION, 1998, 61 (04) :482-486

[9] Identification of sole (Solea solea) and Greenland halibut (Reinhardtius hippoglossoides) by PCR amplification of the 5S rDNA gene [J].

Céspedes, A ;

García, T ;

Carrera, E ;

González, I ;

Fernández, A ;

Hernández, PE ;

Martín, R .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 1999, 47 (03) :1046-1050

[10] BIOCHEMICAL RESEARCH ON OOGENESIS - OOCYTES AND LIVER-CELLS OF TELEOST FISH TINCA-TINCA CONTAIN DIFFERENT KINDS OF 5S RNA [J].

DENIS, H ;

WEGNEZ, M .

DEVELOPMENTAL BIOLOGY, 1977, 59 (02) :228-236

← 1 2 3 4 →