A CRISPR/Cas9 system adapted for gene editing in marine algae

被引：270

作者：

Nymark, Marianne ^{[1
]}

Sharma, Amit Kumar ^{[1
]}

Sparstad, Torfinn ^{[1
]}

Bones, Atle M. ^{[1
]}

Winge, Per ^{[1
]}

机构：

[1] Norwegian Univ Sci & Technol, Dept Biol, N-7491 Trondheim, Norway

来源：

SCIENTIFIC REPORTS | 2016年 / 6卷

关键词：

SIGNAL RECOGNITION PARTICLE; PHAEODACTYLUM-TRICORNUTUM; GENOME; DIATOMS; LIGHT; OCEAN; RICE;

D O I：

10.1038/srep24951

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Here we report that the CRISPR/Cas9 technology can be used to efficiently generate stable targeted gene mutations in microalgae, using the marine diatom Phaeodactylum tricornutum as a model species. Our vector design opens for rapid and easy adaption of the construct to the target chosen. To screen for CRISPR/Cas9 mutants we employed high resolution melting based PCR assays, mutants were confirmed by sequencing and further validated by functional analyses.

引用

页数：6

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