Novel Carbohydrate-Binding Module Identified in a Ruminal Metagenomic Endoglucanase

被引：12

作者：

Duan, Cheng-Jie ^{[1
,2
,3
,4
]}

Liu, Jun-Liang ^{[1
,2
,3
,4
]}

Wu, Xi ^{[1
,2
]}

Tang, Ji-Liang ^{[1
,2
]}

Feng, Jia-Xun ^{[1
,2
,3
,4
]}

机构：

[1] Guangxi Univ, Coll Life Sci & Technol, Nanning 530004, Guangxi, Peoples R China

[2] Guangxi Univ, Guangxi Key Lab Subtrop Bioresource Conservat & U, Key Lab, Minist Educ Microbial & Plant Genet Engn, Nanning 530004, Guangxi, Peoples R China

[3] Guangxi Acad Sci, Natl Engn Res Ctr Nonfood Biorefinery, Cellulose Proc Lab, Nanning 530003, Guangxi, Peoples R China

[4] Guangxi Acad Sci, Guangxi Key Lab Bioind Technol, Nanning 530003, Guangxi, Peoples R China

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2010年 / 76卷 / 14期

基金：

中国国家自然科学基金;

关键词：

CLOSTRIDIUM-THERMOCELLUM; CELLULOSE;

D O I：

10.1128/AEM.00011-10

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Endoglucanase C5614-1 comprises a catalytic module (CM) and an X module (XM). The XM showed no significant homology with known carbohydrate-binding modules (CBMs). Recombinant full-length endoglucanase could bind Avicel, whereas the CM could not. The XM could bind various polysaccharides. The results demonstrated that the XM was a new CBM.

引用

页码：4867 / 4870

页数：4

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