Analysis of immunodominant piroplasm surface protein genes of benign Theileria parasites distributed in China and Korea by allele-specific polymerase chain reaction
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作者:
Kakuda, T
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机构:Hokkaido Univ, Grad Sch Vet Med, Sapporo, Hokkaido 060, Japan
Kakuda, T
Kubota, S
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机构:Hokkaido Univ, Grad Sch Vet Med, Sapporo, Hokkaido 060, Japan
Kubota, S
Sugimoto, C
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机构:Hokkaido Univ, Grad Sch Vet Med, Sapporo, Hokkaido 060, Japan
Sugimoto, C
Baek, BK
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机构:Hokkaido Univ, Grad Sch Vet Med, Sapporo, Hokkaido 060, Japan
Baek, BK
Yin, H
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机构:Hokkaido Univ, Grad Sch Vet Med, Sapporo, Hokkaido 060, Japan
Yin, H
Onuma, M
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机构:Hokkaido Univ, Grad Sch Vet Med, Sapporo, Hokkaido 060, Japan
Onuma, M
机构:
[1] Hokkaido Univ, Grad Sch Vet Med, Sapporo, Hokkaido 060, Japan
[2] Kyoto Biken Labs, Div Vet Microbiol, Kyoto 611, Japan
[3] Chonbuku Natl Univ, Coll Vet Med, Chonju 560756, South Korea
[4] Lanzhou Vet Res Inst, Gansu 730046, Peoples R China
Benign Theileria species distributed in China and Korea were characterized by allele-specific polymerase chain reaction (PCR), based on the sequences of major immunodominant piroplasm surface protein genes. In China, all the isolates contained Chitose (C) type parasites. One out of 5 isolates tested was a mixed population of Ikeda (I), C and B-2 types, whereas, all the isolates from Korea consisted of I type parasites. Except for 4 isolates, 29 isolates from Korea consisted of more than two types of parasites. The present data showed that benign Theileria species distributed in these countries were mixed parasite populations.