Upregulation of GRP78 in Renal Cell Carcinoma and Its Significance

被引：36

作者：

Fu, Weijin ^{[1
]}

Wu, Xiaoyun

Li, Jiachu

Mo, Zengnan

Yang, Zhanbing

Huang, Weihua

Ding, Qiang

机构：

[1] Guangxi Med Univ, Dept Urol, Affiliated Hosp 1, Nanning, Guangxi, Peoples R China

来源：

UROLOGY | 2010年 / 75卷 / 03期

关键词：

ENDOPLASMIC-RETICULUM; PROTEIN GRP78; PROSTATE-CANCER; STRESS-RESPONSE; POOR-PROGNOSIS; ER STRESS; EXPRESSION; METASTASIS; ACTIVATION; APOPTOSIS;

D O I：

10.1016/j.urology.2009.05.007

中图分类号：

R5 [内科学]; R69 [泌尿科学（泌尿生殖系疾病）];

学科分类号：

1002 ; 100201 ;

摘要：

OBJECTIVES To investigate the expression of GRP78 in human renal cell carcinoma (RCC) and its significance. METHODS We studied RNA and tissue section of a tumor and adjacent nontumorous renal tissues obtained from radical nephrectomy specimens of 42 patients and RCC cell lines. We used reverse transcriptase-PCR and immunohistochemistry to detect the GRP78 mRNA and protein expression, respectively. RESULTS Reverse transcriptase-PCR revealed that GRP78 mRNA is positively expressed in RCC cell lines (786-0, OS-RC-2, and Caki-1); the GRP78 mRNA expression in the RCC and adjacent nontumorous renal tissues was 0.88 +/- 0.34 and 0.44 +/- 0.15, respectively (P < .001). The GRP78 protein was found in RCC cell lines. Immunohistochemistry results also showed that the level of GRP78 protein expression of RCC tissues was significantly higher than that of the adjacent nontumorous renal tissues (P < .001). The high levels of GRP78 mRNA and protein expression were related to the large tumor size and high clinical stage (P < .001) but not to sex, age, and cell differentiate (P < .05). CONCLUSIONS To our knowledge, the present study is the first to report the upregulated expression of GRP78 and that it is possibly involved in pathogenesis of RCC. UROLOGY 75: 603-607, 2010. (C) 2010 Elsevier Inc.

引用

页码：603 / 607

页数：5

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