Short-Chain Fructooligosaccharide Regulates Hepatic Peroxisome Proliferator-Activated Receptor α and Farnesoid X Receptor Target Gene Expression in Rats

被引：15

作者：

Fukasawa, Tomoyuki ^{[1
]}

Kamei, Asuka ^{[2
]}

Watanabe, Yuki ^{[2
]}

Koga, Jinichiro ^{[1
]}

Abe, Keiko ^{[3
]}

机构：

[1] Meiji Seika Kaisha Ltd, Food & Hlth R&D Labs, Sakado, Saitama 3500289, Japan

[2] Kanagawa Acad Sci & Technol, Food Safety & Rehabil Project, Takatsu Ku, Kawasaki, Kanagawa 2130012, Japan

[3] Univ Tokyo, Grad Sch Agr & Life Sci, Dept Appl Biol Chem, Bunkyo Ku, Tokyo 1138657, Japan

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2010年 / 58卷 / 11期

关键词：

Fructooligosaccharide; nutrigenomics; DNA microarray; PPAR alpha and FXR target genes; SALT EXPORT PUMP; NUCLEAR RECEPTOR; FXR AGONISTS; MARKER GENES; PPAR-ALPHA; BILE; IDENTIFICATION; NUTRIGENOMICS; MODULATION; SWEETENER;

D O I：

10.1021/jf1006616

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

Prebiotic short-chain fructooligosaccharide (scFOS) is known to have various beneficial effects in humans and animals. Using a nutrigenomic approach, we have previously identified marker genes for the intestinal immunomodulatory and lipid-lowering effects of scFOS. The present study aimed to predict novel physiological effects of scFOS through nutrigenomic analyses. DNA microarray analysis revealed that administration of scFOS changed the expression of the nuclear receptors peroxisome proliferator-activated receptor alpha (PPAR alpha) and farnesoid X receptor (FXR) target genes in the rat liver. Gene expression analysis provided some new interesting hypotheses, for instance, the possible improvement of bile secretion via FXR target genes, and regulation of amino acid metabolism and the urea cycle via PPARa and/or FXR target genes. Our findings clearly indicated that nutrigenomics may make it possible to screen for novel physiological effects of dietary ingredients.

引用

页码：7007 / 7012

页数：6

共 51 条

[1]

ABE K, 2006, SCI TECHNOL JPN, V99, P10

[2]

ADACHI T, 1983, JAPAN FOOD SCI, V22, P71

[3] Conformationally constrained farnesoid X receptor (FXR) agonists: Naphthoic acid-based analogs of GW 4064 [J].

Akwabi-Ameyaw, Adwoa ;

Bass, Jonathan Y. ;

Caldwell, Richard D. ;

Caravella, Justin A. ;

Chen, Lihong ;

Creech, Katrina L. ;

Deaton, David N. ;

Jones, Stacey A. ;

Kaldor, Istvan ;

Liu, Yaping ;

Madauss, Kevin P. ;

Marr, Harry B. ;

McFadyen, Robert B. ;

Miller, Aaron B. ;

Navas, Frank, III ;

Parks, Derek J. ;

Spearing, Paul K. ;

Todd, Dan ;

Williams, Shawn P. ;

Wisely, G. Bruce .

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, 2008, 18 (15) :4339-4343

[4] Human bile salt export pump promoter is transactivated by the farnesoid X receptor/bile acid receptor [J].

Ananthanarayanan, M ;

Balasubramanian, N ;

Makishima, M ;

Mangelsdorf, DJ ;

Suchy, FJ .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2001, 276 (31) :28857-28865

[5] Functional food science and food for specified health use policy in Japan: state of the art [J].

Arai, Soichi ;

Yasuoka, Akihito ;

Abe, Keiko .

CURRENT OPINION IN LIPIDOLOGY, 2008, 19 (01) :69-73

[6]

Bird A R, 2000, Curr Issues Intest Microbiol, V1, P25

[7] Rank products: a simple, yet powerful, new method to detect differentially regulated genes in replicated microarray experiments [J].

Breitling, R ;

Armengaud, P ;

Amtmann, A ;

Herzyk, P .

FEBS LETTERS, 2004, 573 (1-3) :83-92

[8] The prebiotic characteristics of fructooligosaccharides are necessary for reduction of TNBS-induced colitis in rats [J].

Cherbut, C ;

Michel, C ;

Lecannu, G .

JOURNAL OF NUTRITION, 2003, 133 (01) :21-27

[9]

Collins MD, 1999, AM J CLIN NUTR, V69, p1052S, DOI 10.1093/ajcn/69.5.1052s

[10]

Crittenden RG, 1999, PROBIOTICS: A CRITICAL REVIEW, P141

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