Enterocin B, a new bacteriocin from Enterococcus faecium T136 which can act synergistically with enterocin A

被引:306
作者
Casaus, P
Nilsen, T
Cintas, LM
Nes, IF
Hernandez, PE
Holo, H
机构
[1] AGR UNIV NORWAY, LAB MICROBIAL GENE TECHNOL, N-1432 AS, NORWAY
[2] UNIV COMPLUTENSE MADRID, FAC VET, DEPT NUTR & BROMATOL 3, E-28040 MADRID, SPAIN
来源
MICROBIOLOGY-SGM | 1997年 / 143卷
关键词
synergistic bacteriocins; Enterococcus faecium;
D O I
10.1099/00221287-143-7-2287
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The strain Enterococcus faecium T136 produces two bacteriocins, enterocin A, a member of the pediocin family of baderiocins, and a new bacteriocin termed enterocin B. The N-terminal amino acid sequences of enterocins A and B were determined, and the gene encoding enterocin B was sequenced. The primary translation product was a 71 aa peptide containing a leader peptide of the double-glycine type which is cleaved off to give mature enterocin B of 53 as. Enterocin B does not belong to the pediocin family of bacteriocins and shows strong homology to carnobacteriocin A. However, sequence similarities in their leader peptides and C-termini suggest that enterocin B and carnobacteriocin A are related to bacteriocins of the pediocin family. Enterocins A and B had only slightly different inhibitory spectra, and both were active against a wide range of Gram-positive bacteria, Including listeriae, staphylococci and most lactic acid bacteria tested. Both had bactericidal activities, but survival at a frequency of 10(-4)10(-2) was observed when sensitive cultures were exposed to either bacteriocin. The number of survivors was drastically reduced when a mixture of the two baderiocins was added to the cells.
引用
收藏
页码:2287 / 2294
页数:8
相关论文
共 36 条
[1]   SIMPLE AND RAPID METHOD FOR ISOLATING LARGE PLASMID DNA FROM LACTIC STREPTOCOCCI [J].
ANDERSON, DG ;
MCKAY, LL .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1983, 46 (03) :549-552
[2]  
[Anonymous], CHEM METHODS PROKARY
[3]   Biochemical and genetic characterization of enterocin A from Enterococcus faecium, a new antilisterial bacteriocin in the pediocin family of bacteriocins [J].
Aymerich, T ;
Holo, H ;
Havarstein, LS ;
Hugas, M ;
Garriga, M ;
Nes, IF .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1996, 62 (05) :1676-1682
[4]   INHIBITORY-ACTION OF NISIN AGAINST LISTERIA-MONOCYTOGENES [J].
BENKERROUM, N ;
SANDINE, WE .
JOURNAL OF DAIRY SCIENCE, 1988, 71 (12) :3237-3245
[5]   PEDIOCIN PA-1, A BACTERIOCIN FROM PEDIOCOCCUS-ACIDILACTICI PAC1.0, FORMS HYDROPHILIC PORES IN THE CYTOPLASMIC MEMBRANE OF TARGET-CELLS [J].
CHIKINDAS, ML ;
GARCIAGARCERA, MJ ;
DRIESSEN, AJM ;
LEDEBOER, AM ;
NISSENMEYER, J ;
NES, IF ;
ABEE, T ;
KONINGS, WN ;
VENEMA, G .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1993, 59 (11) :3577-3584
[6]   ISOLATION AND CHARACTERIZATION OF PEDIOCIN L50, A NEW BACTERIOCIN FROM PEDIOCOCCUS-ACIDILACTICI WITH A BROAD INHIBITORY SPECTRUM [J].
CINTAS, LM ;
RODRIGUEZ, JM ;
FERNANDEZ, MF ;
SLETTEN, K ;
NES, IF ;
HERNANDEZ, PE ;
HOLO, H .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1995, 61 (07) :2643-2648
[7]   EVIDENCE THAT THE AMYLOID FIBRIL PROTEIN IN SENILE SYSTEMIC AMYLOIDOSIS IS DERIVED FROM NORMAL PREALBUMIN [J].
CORNWELL, GG ;
SLETTEN, K ;
JOHANSSON, B ;
WESTERMARK, P .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1988, 154 (02) :648-653
[8]  
DAESCHEL MA, 1989, FOOD TECHNOL-CHICAGO, V43, P164
[9]  
DELVESBROUGHTON J, 1990, FOOD TECHNOL-CHICAGO, V44, P100
[10]   A COMPREHENSIVE SET OF SEQUENCE-ANALYSIS PROGRAMS FOR THE VAX [J].
DEVEREUX, J ;
HAEBERLI, P ;
SMITHIES, O .
NUCLEIC ACIDS RESEARCH, 1984, 12 (01) :387-395