Oxidative stress induces various post-translational modifications (PTM); some are reversible in vivo via enzymatic catalysis. The present paper reviews specific procedures for the detection of oxidative PTM in proteins, most of them including electrophoresis. Main topics are carbonylated and glutathionylated proteins as well as modification of selected amino acids ( Cys, Tyr, Met, Trp, Lys).
机构:
Chinese Acad Med Sci, Inst Basic Med Sci, Key Lab Med Mol Biol, Dept Pathophysiol,Sch Basic Med, Beijing 100730, Peoples R ChinaChinese Acad Med Sci, Inst Basic Med Sci, Key Lab Med Mol Biol, Dept Pathophysiol,Sch Basic Med, Beijing 100730, Peoples R China
Liu, Liu
Liu, Xuejiao
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机构:
Capital Med Univ, Beijing Anzhen Hosp, Dept Nephrol, Beijing, Peoples R China
Peking Union Med Coll Hosp, Dept Nephrol, Beijing, Peoples R ChinaChinese Acad Med Sci, Inst Basic Med Sci, Key Lab Med Mol Biol, Dept Pathophysiol,Sch Basic Med, Beijing 100730, Peoples R China
机构:
Univ Sheffield, Acad Nephrol Unit, Med Sch, Kidney Genet Grp, Sheffield, S Yorkshire, EnglandUniv Sheffield, Acad Nephrol Unit, Med Sch, Kidney Genet Grp, Sheffield, S Yorkshire, England