Detecting oxidative post-translational modifications in proteins

被引：41
作者：
Gianazza, E.
Crawford, J.
Miller, I.
机构：
[1] Univ Milan, Dipartimento Sci Farmacol, Grp Studio Proteom & Struttura Prot, I-20133 Milan, Italy
[2] Nonlinear Dynam Ltd, Newcastle Upon Tyne, Tyne & Wear, England
[3] Vet Med Univ Wien, Inst Med Chem, Dept Nat Wissensch, Vienna, Austria
来源：
AMINO ACIDS | 2007年 / 33卷 / 01期
关键词：
oxidative post-translational modifications; proteomics; electrophoresis; carbonylation; glutathionylation;
D O I：
10.1007/s00726-006-0410-2
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Oxidative stress induces various post-translational modifications (PTM); some are reversible in vivo via enzymatic catalysis. The present paper reviews specific procedures for the detection of oxidative PTM in proteins, most of them including electrophoresis. Main topics are carbonylated and glutathionylated proteins as well as modification of selected amino acids ( Cys, Tyr, Met, Trp, Lys).
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页码：51 / 56
页数：6
相关论文
共 63 条
[1]   Protein oxidation biomarkers in hyperoxic lung injury in rats: effects of U-74389 [J].
Awasthi, S ;
Gyurasics, A ;
Knight, SA ;
Welty, SE ;
Smith, CV .
TOXICOLOGY LETTERS, 1998, 95 (01) :47-61
[2]  
Baty JW, 2002, PROTEOMICS, V2, P1261, DOI 10.1002/1615-9861(200209)2:9<1261::AID-PROT1261>3.0.CO
[3]  
2-Q
[4]   Immunological detection and quantification of oxidized proteins by labelling with digoxigenin [J].
Bautista, J ;
Mateos-Nevado, MD .
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 1998, 62 (03) :419-423
[5]   Protein oxidation in aging, disease, and oxidative stress [J].
Berlett, BS ;
Stadtman, ER .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (33) :20313-20316
[6]   Biphasic lindane-induced oxidation of glutathione and inhibition of gap junctions in myometrial cells [J].
Caruso, RL ;
Upham, BL ;
Harris, C ;
Trosko, JE .
TOXICOLOGICAL SCIENCES, 2005, 86 (02) :417-426
[7]   Protein nitration in a mouse model of familial amyotrophic lateral sclerosis - Possible multifunctional role in the pathogenesis [J].
Casoni, F ;
Basso, M ;
Massignan, T ;
Gianazza, E ;
Cheroni, C ;
Salmona, M ;
Bendotti, C ;
Bonetto, V .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2005, 280 (16) :16295-16304
[8]   Reversal of protein S-glutathiolation by glutaredoxin in the retinal pigment epithelium [J].
Chai, YC ;
Hoppe, G ;
Sears, J .
EXPERIMENTAL EYE RESEARCH, 2003, 76 (02) :155-159
[9]   Detection of S-glutathionylated proteins by glutathione S-transferase overlay [J].
Cheng, G ;
Ikeda, Y ;
Iuchi, Y ;
Fujii, J .
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 2005, 435 (01) :42-49
[10]  
Czapski G A, 2001, Med Sci Monit, V7, P606
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