Self-assembled monolayers improve protein distribution on holey carbon cryo-EM supports

被引：69

作者：

Meyerson, Joel R. ^{[1
]}

Rao, Prashant ^{[1
]}

Kumar, Janesh ^{[2
]}

Chittori, Sagar ^{[2
]}

Banerjee, Soojay ^{[1
]}

Pierson, Jason ^{[3
]}

Mayer, Mark L. ^{[2
]}

Subramaniam, Sriram ^{[1
]}

机构：

[1] NCI, Cell Biol Lab, Ctr Canc Res, NIH, Bethesda, MD 20892 USA

[2] NICHD, Lab Cellular & Mol Neurophysiol, Porter Neurosci Res Ctr, NIH, Bethesda, MD 20892 USA

[3] FEI Co, Hillsboro, OR 97124 USA

来源：

SCIENTIFIC REPORTS | 2014年 / 4卷

关键词：

CRYOELECTRON; ADSORPTION; SPECIMENS; GRAPHENE;

D O I：

10.1038/srep07084

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Poor partitioning of macromolecules into the holes of holey carbon support grids frequently limits structural determination by single particle cryo-electron microscopy (cryo-EM). Here, we present a method to deposit, on gold-coated carbon grids, a self-assembled monolayer whose surface properties can be controlled by chemical modification. We demonstrate the utility of this approach to drive partitioning of ionotropic glutamate receptors into the holes, thereby enabling 3D structural analysis using cryo-EM methods.

引用

页数：5

共 33 条

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