Self-assembled monolayers improve protein distribution on holey carbon cryo-EM supports

被引：69

作者：

Meyerson, Joel R. ^{[1
]}

Rao, Prashant ^{[1
]}

Kumar, Janesh ^{[2
]}

Chittori, Sagar ^{[2
]}

Banerjee, Soojay ^{[1
]}

Pierson, Jason ^{[3
]}

Mayer, Mark L. ^{[2
]}

Subramaniam, Sriram ^{[1
]}

机构：

[1] NCI, Cell Biol Lab, Ctr Canc Res, NIH, Bethesda, MD 20892 USA

[2] NICHD, Lab Cellular & Mol Neurophysiol, Porter Neurosci Res Ctr, NIH, Bethesda, MD 20892 USA

[3] FEI Co, Hillsboro, OR 97124 USA

来源：

SCIENTIFIC REPORTS | 2014年 / 4卷

关键词：

CRYOELECTRON; ADSORPTION; SPECIMENS; GRAPHENE;

D O I：

10.1038/srep07084

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Poor partitioning of macromolecules into the holes of holey carbon support grids frequently limits structural determination by single particle cryo-electron microscopy (cryo-EM). Here, we present a method to deposit, on gold-coated carbon grids, a self-assembled monolayer whose surface properties can be controlled by chemical modification. We demonstrate the utility of this approach to drive partitioning of ionotropic glutamate receptors into the holes, thereby enabling 3D structural analysis using cryo-EM methods.

引用

页数：5

共 33 条

[1] An Investigation of the Effects of Self-Assembled Monolayers on Protein Crystallisation
Zhang, Chen-Yan
Shen, He-Fang
Wang, Qian-Jin
Guo, Yun-Zhu
He, Jin
Cao, Hui-Ling
Liu, Yong-Ming
Shang, Peng
Yin, Da-Chuan
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2013, 14 (06): : 12329 - 12345
[2] Pragmatic studies on protein-resistant self-assembled monolayers
Hahn, Christoph D.
Tinazli, Ali
Hoelzl, Martin
Leitner, Christa
Frederix, Filip
Lackner, Bernd
Mueller, Norbert
Klampfl, Christian
Tampe, Robert
Gruber, Hermann J.
MONATSHEFTE FUR CHEMIE, 2007, 138 (03): : 245 - 252
[3] Membrane protein resistance of oligo(ethylene oxide) self-assembled monolayers
Vaish, Amit
Vanderah, David J.
Vierling, Ryan
Crawshaw, Fay
Gallagher, D. Travis
Walker, Marlon L.
COLLOIDS AND SURFACES B-BIOINTERFACES, 2014, 122 : 552 - 558
[4] Functionalization of gold-coated carbon nanotubes with self-assembled monolayers of thiolates
Ceglowski, Michal
Narkiewicz, Urszula
Pelech, Iwona
Schroeder, Grzegorz
JOURNAL OF MATERIALS SCIENCE, 2012, 47 (07) : 3463 - 3467
[5] Carbon nanomembranes from self-assembled monolayers: Functional surfaces without bulk
Turchanin, Andrey
Goelzhaeuser, Armin
PROGRESS IN SURFACE SCIENCE, 2012, 87 (5-8) : 108 - 162
[6] Protein blocking inhibits ambient degradation of self-assembled monolayers for affinity biosensing
Brightbill, Eleanor L.
Gezahagne, Hilena F.
Jin, Decarle S.
Brown, Billyde
Vogel, Eric M.
APPLIED SURFACE SCIENCE, 2021, 557
[7] Removal of Co, Sr and Cs from aqueous solution using self-assembled monolayers on mesoporous supports
Park, Younjin
Shin, Won Sik
Choi, Sang-June
KOREAN JOURNAL OF CHEMICAL ENGINEERING, 2012, 29 (11) : 1556 - 1566
[8] The use of mixed self-assembled monolayers as a strategy to improve the efficiency of carbamate detection in environmental monitoring
Cancino, Juliana
Razzino, Claudia A.
Zucolotto, Valtencir
Machado, Sergio A. S.
ELECTROCHIMICA ACTA, 2013, 87 : 717 - 723
[9] Protein Density Profile at the Interface of Water with Oligo(ethylene glycol) Self-Assembled Monolayers
Skoda, M. W. A.
Schreiber, F.
Jacobs, R. A. J.
Webster, J. R. P.
Wolff, M.
Dahint, R.
Schwendel, D.
Grunze, M.
LANGMUIR, 2009, 25 (07) : 4056 - 4064
[10] Controlled Modification of Protein-Repelling Self-Assembled Monolayers by Ultraviolet Light: The Effect of the Wavelength
Jeyachandran, Y. L.
Terfort, Andreas
Zharnikov, Michael
JOURNAL OF PHYSICAL CHEMISTRY C, 2012, 116 (16) : 9019 - 9028

← 1 2 3 4 →