Callus induction and somatic embryogenesis of Phalaenopsis

被引：107

作者：

Ishii, Y ^{[1
]}

Takamura, T ^{[1
]}

Goi, M ^{[1
]}

Tanaka, M ^{[1
]}

机构：

[1] Kagawa Univ, Fac Agr, Dept Hort, Miki, Kagawa 7610795, Japan

来源：

PLANT CELL REPORTS | 1998年 / 17卷 / 6-7期

关键词：

Phalaenopsis; protocorm-like bodies; callus induction; somatic embryogenesis;

D O I：

10.1007/s002990050423

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Callus induction and plant regeneration through somatic embryogenesis in Phalaenopsis Richard Shaffer 'Santa Cruz' were examined. Protocorm-like body (PLB) segments formed calli in Vacin and Went medium with sucrose. The optimal concentration of sucrose was 40 g.l(-1). Medium containing 200 ml.l(-1) coconut water together with 40 g.l(-1) sucrose was effective for callus induction. Gellan gum was suitable than agar as a gelling agent for callus induction. The calli easily formed PLBs after being transferred to a medium without sucrose. Histological observation suggested that the PLBs were somatic embryos. No variation was observed in the flowering plants regenerated through somatic embryogenesis.

引用

页码：446 / 450

页数：5

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