Effect of MTAD on the Differentiation of Osteoblast-like Cells

Introduction: The aim of the present study was to investigate the effect of MTAD on the differentiation of osteoblast-like cells. Methods: The cell viability assay was performed to evaluate the cytotoxicity of MTAD on MC3T3-E1 and periodontal ligament cells at the various concentrations. The bone sialoprotein (BSP) gene expression was also examined by real-time polymerase chain reaction. Results: MTAD exhibited a lower cytotoxicity compared with other intracanal irrigants and medication. The MC3T3-E1 cells treated with H(2)O(2) showed a decrease in the alkaline phosphatase (ALP) activity by 40% on day 14 compared with the control group at the concentration of 50 mu g/mIL. No significant difference in the ALP activity was observed between MTAD and control group. Furthermore, MTAD and Ca(OH)(2) paste did not change in BSP gene expression in MC3T3-E1 cells on day 21. Conclusions: These results suggested that MTAD is a less cytotoxic irrigant and does not affect differentiation into osteoblasts compared with other intracanal irrigants, such as H(2)O(2), NaOl, ethylenediaminetetraacetic acid, and chlorhexidine. (J Endod 2010;36:260-263)