Single. amino acid changes in the turnip mosaic virus viral genome-linked protein (VPg) confer virulence towards Arabidopsis thaliana mutants knocked out for eukaryotic initiation factors eIF(iso)4E and elF(iso)4G

被引：64
作者：
Gallois, Jean-Luc [1 ]
Charron, Carine [1 ]
Sanchez, Flora [2 ]
Pagny, Gaelle [3 ]
Houvenaghel, Marie-Christine [3 ]
Moretti, Andre [1 ]
Ponz, Fernando [2 ]
Revers, Frederic [3 ]
Caranta, Carole [1 ]
German-Retana, Sylvie [3 ]
机构：
[1] INRA, Genet & Breeding Fruits & Vegetable, F-84143 Montfavet, France
[2] UPM INIA, CBGP, Madrid 28223, Spain
[3] Univ Bordeaux 2, INRA, IPV, UMR GDPP 1090, F-33883 Villenave Dornon, France
来源：
JOURNAL OF GENERAL VIROLOGY | 2010年 / 91卷
关键词：
CAP-INDEPENDENT TRANSLATION; TOBACCO-ETCH-VIRUS; RESISTANCE GENE; POTYVIRUS INFECTION; BINDING PROTEIN; CODING REGION; FACTOR EIF4E; 4E; INVOLVEMENT; MUTATIONS;
D O I：
10.1099/vir.0.015321-0
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Previous resistance analyses of Arabidopsis thaliana mutants knocked out for eukaryotic translation initiation factors showed that disruption of the At-elF(isc)4E or both the Ate1F(iso)4G1 and At-elF(iso)4G2 genes resulted in resistance against turnip mosaic virus (TuMV). This study selected TuMV virulent variants that overcame this resistance and showed that two independent mutations in the region coding for the viral genome-linked protein (VPg) were sufficient to restore TuMV virulence in At-elF(isc)4E and At-e1F(iso)4G1xAt-elF(iso)4G2 knockout plants. As a VPg-elF(iso)4E interaction has been shown previously to be critical for TuMV infection, a systematic analysis of the interactions between A. thatiana elF4Es and VPgs of virulent and avirulent TuMVs was performed. The results suggest that virulent TuMV variants may use an elF4F-independent pathway.
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页码：288 / 293
页数：6
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