The use of lysozyme modified with fluorescein for the detection of Gram-positive bacteria

被引：20

作者：

Arabski, Michal ^{[1
]}

Konieczna, Iwona ^{[1
]}

Tusinska, Ewa ^{[1
]}

Wasik, Slawomir ^{[2
]}

Relich, Inga ^{[3
]}

Zajac, Krzysztof ^{[3
]}

Kaminski, Zbigniew J. ^{[3
]}

Kaca, Wieslaw ^{[1
]}

机构：

[1] Jan Kochanowski Univ Kielce, Dept Microbiol, PL-25406 Kielce, Poland

[2] Jan Kochanowski Univ, Dept Mol Phys, PL-25406 Kielce, Poland

[3] Tech Univ Lodz, Inst Organ Chem, PL-90924 Lodz, Poland

来源：

MICROBIOLOGICAL RESEARCH | 2015年 / 170卷

关键词：

Lysozyme; Fluorescein; Laser interferometry; Gram positive bacteria; SOIL; AMPICILLIN; DIVERSITY; BINDING; ACID; DNA;

D O I：

10.1016/j.micres.2014.05.004

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Lysozyme (1,4-beta-N-acetylmuramidase) is commonly applied in the food, medical, and pharmaceutical industries. In this study, we tested a novel application of fluorescein-modified lysozyme (using carboxyfluorescein with a triazine-based coupling reagent) as a new tool for the detection of Gram-positive soil bacteria. The results, obtained by cultivation methods, fluorescence analysis, and laser interferometry, showed that, after optimization, fluorescein-modified lysozyme could be used to evaluate the prevalence of Gram-positive bacteria essential in bioremediation of soils with low pH, such as those degraded by sulfur. (C) 2014 Elsevier GmbH. All rights reserved.

引用

页码：242 / 247

页数：6

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