Effect of gamma-ray irradiation on Escherichia coli motility

被引：4

作者：

Atsumi, Tatsuo ^{[1
]}

Fujimoto, Eriko ^{[2
]}

Furuta, Masakazu ^{[3
]}

Kato, Mikio ^{[2
,4
]}

机构：

[1] Gifu Univ Med Sci, Sch Hlth Sci, Dept Radiol Technol, Seki 5013892, Japan

[2] Osaka Prefecture Univ, Sch Sci, Dept Biol Sci, Sakai, Osaka 5998531, Japan

[3] Osaka Prefecture Univ, Grad Sch Engn, Radiat Res Ctr, Dept Quantum & Radiat Engn, Sakai, Osaka 5998531, Japan

[4] Osaka Prefecture Univ, Fac Liberal Arts & Sci, Dept Biol, Sakai, Osaka 5998531, Japan

来源：

CENTRAL EUROPEAN JOURNAL OF BIOLOGY | 2014年 / 9卷 / 10期

关键词：

Escherichia coli; Flagellar motor; Gamma rays; Ionizing radiation; Motility; BACTERIAL FLAGELLAR MOTORS; FORCE-GENERATING UNITS; RADIATION-INACTIVATION; ROTARY MOTOR; E; COLI; PROTEIN; O157H7; TORQUE;

D O I：

10.2478/s11535-014-0332-z

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The effects of ionizing radiation on bacteria are generally evaluated from the dose-dependent survival ratio, which is determined by colony-forming ability and mutation rate. The mutagenic damage to cellular DNA induced by radiation has been extensively investigated; however, the effects of irradiation on the cellular machinery in situ remain unclear. In the present work, we irradiated Escherichia coli cells in liquid media with gamma rays from Co-60 (in doses up to 8 kGy). The swimming speeds of the cells were measured using a microscope. We found that the swimming speed was unaltered in cells irradiated with a lethal dose of gamma rays. However, the fraction of motile cells decreased in a dose-dependent manner. Similar results were observed when protein synthesis was inhibited by treatment with kanamycin. Evaluation of bacterial swimming speed and the motile fraction after irradiation revealed that some E. coli cells without the potential of cell growth and division remained motile for several hours after irradiation.

引用

页码：909 / 914

页数：6

共 23 条

[1] NONCHEMOTACTIC MUTANTS OF ESCHERICHIA COLI [J].

ARMSTRONG, JB ;

ADLER, J ;

DAHL, MM .

JOURNAL OF BACTERIOLOGY, 1967, 93 (01) :390-+

[2] An ultrasonic motor model for bacterial flagellar motors [J].

Atsumi, T .

JOURNAL OF THEORETICAL BIOLOGY, 2001, 213 (01) :31-51

[3] The rotary motor of bacterial flagella [J].

Berg, HC .

ANNUAL REVIEW OF BIOCHEMISTRY, 2003, 72 :19-54

[4] RESTORATION OF TORQUE IN DEFECTIVE FLAGELLAR MOTORS [J].

BLAIR, DF ;

BERG, HC .

SCIENCE, 1988, 242 (4886) :1678-1681

[5]

BLOCK SM, 1984, NATURE, V309, P470, DOI 10.1038/309470a0

[6] Protein dynamics and mechanisms controlling the rotational behaviour of the bacterial flagellar motor [J].

Brown, Mostyn T. ;

Delalez, Nicolas J. ;

Armitage, Judith P. .

CURRENT OPINION IN MICROBIOLOGY, 2011, 14 (06) :734-740

[7] Radiation-induced damage to cellular DNA: measurement and biological role [J].

Cadet, J ;

Douki, T ;

Gasparutto, D ;

Ravanat, JL .

RADIATION PHYSICS AND CHEMISTRY, 2005, 72 (2-3) :293-299

[8] Molecular characterization and functional analysis by heterologous expression in E. coli under diverse abiotic stresses for OsLEA5, the atypical hydrophobic LEA protein from Oryza sativa L. [J].

He, Shuai ;

Tan, Lili ;

Hu, Zongli ;

Chen, Guoping ;

Wang, Guixue ;

Hu, Tingzhang .

MOLECULAR GENETICS AND GENOMICS, 2012, 287 (01) :39-54

[9] Glass capillary optics for producing nanometer sized beams and its applications [J].

Ikeda, Tokihiro ;

Kanai, Yasuyuki ;

Iwai, Yoshio ;

Kojima, Takao M. ;

Maeshima, Kazuhiro ;

Meissl, Walter ;

Kobayashi, Tomohiro ;

Nebiki, Takuya ;

Miyamoto, So ;

Pokhil, Grigory P. ;

Narusawa, Tadashi ;

Imamoto, Naoko ;

Yamazaki, Yasunori .

SURFACE & COATINGS TECHNOLOGY, 2011, 206 (05) :859-863

[10] Inactivation of Escherichia coli O157:H7 on Lettuce, Using Low-Energy X-Ray Irradiation [J].

Jeong, Sanghyup ;

Marks, Bradley P. ;

Ryser, Elliot T. ;

Moosekian, Scott R. .

JOURNAL OF FOOD PROTECTION, 2010, 73 (03) :547-551

← 1 2 3 →