Evaluation of ABCG2 and p63 expression in canine cornea and cultivated corneal epithelial cells

被引：32

作者：

Morita, Maresuke ^{[1
]}

Fujita, Naoki ^{[1
]}

Takahashi, Ayaka ^{[1
]}

Nam, Eun Ryel ^{[1
]}

Yui, Sho ^{[1
]}

Chung, Cheng Shu ^{[1
]}

Kawahara, Naoya ^{[1
]}

Lin, Hsing Yi ^{[1
]}

Tsuzuki, Keiko ^{[1
]}

Nakagawa, Takayuki ^{[1
]}

Nishimura, Ryohei ^{[1
]}

机构：

[1] Univ Tokyo, Grad Sch Agr & Life Sci, Lab Vet Surg, Bunkyo Ku, Tokyo 1138657, Japan

来源：

VETERINARY OPHTHALMOLOGY | 2015年 / 18卷 / 01期

关键词：

ABCG2; canine corneal epithelium; corneal epithelial stem cells; dog; limbus; p63; SIDE-POPULATION CELLS; HUMAN LIMBAL; STEM-CELLS; MARKERS; DIFFERENTIATION; LOCATION;

D O I：

10.1111/vop.12147

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

ObjectiveTo examine the expressions of ABCG2 and p63 in canine corneal epithelia and to evaluate their significance in corneal regeneration. ProceduresCanine corneal and limbal epithelial cells were obtained from five healthy beagle dogs. We analyzed the morphological properties of cultivated limbal and corneal epithelial cells. We compared the expressions of ABCG2 and p63 in the limbus and central cornea by immunohistochemistry and real-time quantitative PCR. We analyzed the expression of these markers in cultivated cells by immunocytochemistry and real-time quantitative PCR. ResultsThe limbal epithelial cells were smaller and proliferated more rapidly than the corneal epithelial cells in primary cultures. The corneal cells failed to be subcultured, whereas the limbal cells could be subcultured with increasing cell size. ABCG2 was localized in the basal layer of the limbal epithelium, and p63 was widely detected in the entire corneal epithelia. ABCG2 expression was significantly higher, and p63 was slightly higher in the limbus compared with the central cornea. ABCG2 was detected only in limbal cells in primary culture, not in corneal cells or passaged limbal cells. p63 was detected in both limbal and corneal cells and decreased gradually in the limbal cells with the cell passages. ConclusionsABCG2 was localized in canine limbal epithelial cells, and p63 was widely expressed in canine corneal epithelia. ABCG2 and p63 could prove to be useful markers in dogs for putative corneal epithelial stem cells and for corneal epithelial cell proliferation, respectively.

引用

页码：59 / 68

页数：10

共 30 条

[1] High expression of p63 combined with a large N/C ratio defines a subset of human limbal epithelial cells: Implications on epithelial stem cells [J].