Recovery of phenolic compounds of food concern from Arthrospira platensis by green extraction techniques

被引:28
作者
da Silva, Milena Fernandes [1 ,2 ]
Casazza, Alessandro Alberto [2 ]
Ferrari, Pier Francesco [2 ]
Aliakbarian, Bahar [2 ]
Converti, Attilio [2 ]
Bezerra, Raquel Pedrosa [3 ]
Figueiredo Porto, Ana Lucia [3 ]
Perego, Patrizia [2 ]
机构
[1] Univ Fed Pernambuco, Biosci Ctr, Ave Prof Moraes Rego 1235,Cidade Univ, BR-50670420 Recife, PE, Brazil
[2] Univ Genoa, Dept Civil Chem & Environm Engn DICCA, Via Opera Pia 15, I-16145 Genoa, Italy
[3] Univ Fed Rural Pernambuco, Dept Morphol & Anim Physiol, Ave Dom Manoel Medeiros S-N, BR-52171900 Recife, PE, Brazil
来源
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS | 2017年 / 25卷
关键词
Arthrospira platensis; Green extraction; Phenolic compounds; Antioxidants; Response surface methodology; High pressure/temperature extraction; SPIRULINA-PLATENSIS; ANTIOXIDANT ACTIVITY; TEMPERATURE EXTRACTION; ASSISTED EXTRACTION; SOLVENT-EXTRACTION; LIQUID EXTRACTION; MICROALGAE; GRAPE; ULTRASOUND; PRESSURE;
D O I
10.1016/j.algal.2017.05.027
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Arthrospira platensis UTEX 1926 has attracted attention because of its ability to produce high value-added compounds such as polyphenols, but their profitable recovery is recognized as a big challenge. In this study, different green extraction techniques, namely ultrasound-assisted (UAE), microwave-assisted (MAE) and high pressure/temperature (HPTE) extractions were compared with classic solid-liquid extraction (SLE) in terms of phenolic compounds recovery from A. platensis using ethanol as solvent and antiradical power (ARP). HPTE proved the most efficient extraction method, allowing maximum total polyphenol yield (TP) of 3.32 +/- 0.08 mg of gallic acid equivalent [GAE] per gram of dry biomass [DB] and ARP of 58.30 +/- 0.12 mu molTrolox g(DB)(-1), whereas maximum total flavonoid yield (TF) was 2.80 +/- 0.18 mg of catechin equivalent [CE] g(DB)(-1) in MAE extract. Once HPTE had been selected as the best-performing extraction method, a 3(2)-full factorial design was applied to evaluate the combined effects of temperature (90 <= T <= 180 degrees C) and ethanol concentration in water (20 <= Sc <= 100% v/v) on TP, TF and ARP by Response Surface Methodology (RSM). RSM revealed that the most suitable conditions for TP (26.00-28.04 mgGAE g(DB)(-1)) and TF (10.25 +/- 0.34 mgCE g(DB)(-1)) were T = 180 degrees C and 20 <= Sc <= 60% (v/v), while ARP was maximized (67.77-69.02 mu molTrolox g(DB)(-1)) at 90 <= T <= 135 degrees C. HPLC analysis showed that catechin, vanillic, gallic and syringic acids were present in very low concentrations (up to 0.05 +/- 0.01 mg 100 g(DB)(-1)) in MAE, UAE and SLE extracts, while they were the most abundant phenolics in HPTE (3.45-3.61, 1.06-2.02, 1.64-1.71 and 0.99-1.26 mg 100 g(DB)(-1), respectively). These compounds taken together effectively contributed to both TP (r = 0.928) and TF (r = 0.960), but not to ARP. This work opens new perspectives for HPTE use as emerging technique to obtain high-ARP phenolic-rich hydroalcoholic extracts of A. platensis, which may serve as a natural source of compounds to formulate functional foods or prepare dietary supplements.
引用
收藏
页码:391 / 401
页数:11
相关论文
共 71 条
  • [1] Abd El-Aty AM, 2014, J APPL PHARM SCI, V4, P069, DOI DOI 10.7324/JAPS.2014.40712
  • [2] Requirements for a cocitation similarity measure, with special reference to Pearson's correlation coefficient
    Ahlgren, P
    Jarneving, B
    Rousseau, R
    [J]. JOURNAL OF THE AMERICAN SOCIETY FOR INFORMATION SCIENCE AND TECHNOLOGY, 2003, 54 (06): : 550 - 560
  • [3] Valorization of olive oil solid waste using high pressure-high temperature reactor
    Aliakbarian, Bahar
    Casazza, Alessandro A.
    Perego, Patrizia
    [J]. FOOD CHEMISTRY, 2011, 128 (03) : 704 - 710
  • [4] Phenolics extraction from Agave americana (L.) leaves using high-temperature, high-pressure reactor
    Ben Hamissa, Aicha Menyar
    Seffen, Mongi
    Aliakbarian, Bahar
    Casazza, Alessandro Alberto
    Perego, Patrizia
    Converti, Attilio
    [J]. FOOD AND BIOPRODUCTS PROCESSING, 2012, 90 (C1) : 17 - 21
  • [5] Properties of lysozyme/Arthrospira platensis (Spirulina) protein complexes for antimicrobial edible food packaging
    Benelhadj, Sonda
    Fejji, Nawel
    Degraeve, Pascal
    Attia, Hamadi
    Ghorbel, Dorra
    Gharsallaoui, Adem
    [J]. ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS, 2016, 15 : 43 - 49
  • [6] Effect of pH on the functional properties of Arthrospira (Spirulina) platensis protein isolate
    Benelhadj, Sonda
    Gharsallaoui, Adem
    Degraeve, Pascal
    Attia, Hamadi
    Ghorbel, Dorra
    [J]. FOOD CHEMISTRY, 2016, 194 : 1056 - 1063
  • [7] Response surface methodology (RSM) as a tool for optimization in analytical chemistry
    Bezerra, Marcos Almeida
    Santelli, Ricardo Erthal
    Oliveira, Eliane Padua
    Villar, Leonardo Silveira
    Escaleira, Luciane Amlia
    [J]. TALANTA, 2008, 76 (05) : 965 - 977
  • [8] Study of solid-liquid extraction kinetics of total polyphenols from grape seeds
    Bucic-Kojic, Ana
    Planinic, Mirela
    Tomas, Srecko
    Bilic, Mate
    Velic, Darko
    [J]. JOURNAL OF FOOD ENGINEERING, 2007, 81 (01) : 236 - 242
  • [9] INFLUENCE OF HIGH-PRESSURE/HIGH-TEMPERATURE EXTRACTION ON THE RECOVERY OF PHENOLIC COMPOUNDS FROM BARLEY GRAINS
    Bucic-Kojic, Ana
    Casazza, Alessandro A.
    Strelec, Ivica
    Paini, Marco
    Planinic, Mirela
    Perego, Patrizia
    [J]. JOURNAL OF FOOD BIOCHEMISTRY, 2015, 39 (06) : 696 - 707
  • [10] Polyphenols from Grape and Apple Skin: a Study on Non-Conventional Extractions and Biological Activity on Endothelial Cell Cultures
    Casazza, Alessandro A.
    Aliakbarian, Bahar
    Mura, Marzia
    Chasseur, Monica
    Freguglia, Marcello
    Valentini, Sabina
    Palombo, Domenico
    Perego, Patrizia
    [J]. FRUTIC ITALY 2015: 9TH NUT AND VEGETABLE PRODUCTION ENGINEERING SYMPOSIUM, 2015, 44 : 205 - 210