The use of nano-fertilizers is an effective alternative to traditional fertilizers, as it achieves many advantages due to its use in smaller quantities, play an important role in plant nutrition and increase the ability of crops to stress through the use of plant growth-promoting (PGP) and phosphates solubilizing bacteria (PSB). A field experiment was carried out at Ras Sudr Research Station of the Desert Research Center. The experiments were conducted to study the effect of nano phosphozink at (50, 100 and 200 ppm) and some phosphate solubilizing bacteria on growth, chemical composition and productivity of Barley in calcareous soil, Treatments were arranged in split plot design with three replicates. The result revealed that, foliar application of Nanophosphozink fertilizer, showed a significantly higher increase of yield parameters of Barley (dry weights of biological yields, straw, grain and plant height) and nutrient contents. Phosphate solubilizing bacteriaas soil drench application (Brevundimonasolei and Acinetobacter baumannii) a repositive to produce proline, organic acids, phosphatase enzymes, catalease enzyme, peroxidase enzyme. They produced higher yields compared with other treatments. The most effective treatment was 200 ppm of Nano phosphozink with Acinetobacter baumannii resulted significant and higher values in this respect compared with other treatments. It could be recommended that Nano phosphozink and the biofertilizer alleviate the hazardous effects of either soil or water salinity, which negatively affect barley seed yield and quality.