Probiotics-Derived Extracellular Vesicles Protect Oxidative Stress against H2O2 Induction in Placental Cells

被引:8
|
作者
Wang, Le-Ming [1 ,2 ]
Lee, Bao-Hong [3 ]
Hou, Chih-Yao [4 ]
Hsu, Wei-Hsuan [5 ,6 ]
Tai, Chen-Jei [1 ,7 ,8 ]
机构
[1] Taipei Med Univ, Coll Med, Grad Inst Clin Med, 250 Wuxing St, Taipei 11031, Taiwan
[2] Taipei Med Univ, Wan Fang Hosp, Dept Obstet & Gynecol, 111,Sec 3,Xinglong Rd, Taipei 11696, Taiwan
[3] Natl Chiayi Univ, Dept Hort, Chiayi 600355, Taiwan
[4] Natl Kaohsiung Univ Sci & Technol, Dept Seafood Sci, Kaohsiung 811213, Taiwan
[5] Natl Cheng Kung Univ, Coll Med, Dept Food Safety Hyg & Risk Management, Tainan 701401, Taiwan
[6] Natl Cheng Kung Univ, Ctr Allergy & MucosaImmun Adv, Tainan 701401, Taiwan
[7] Taipei Med Univ, Coll Med, Sch Med, Dept Obstet & Gynecol, Taipei 11042, Taiwan
[8] Taipei Med Univ Hosp, Dept Chinese Med, Taipei 11042, Taiwan
来源
FERMENTATION-BASEL | 2022年 / 8卷 / 02期
关键词
spontaneous preterm birth (sPTB); Lactobacillus crispatus; extracellular vesicles (EVs); senescence; mitochondrial fusion; PRETERM BIRTH; PREDICTION; INFECTION;
D O I
10.3390/fermentation8020074
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
(1) Background: The microbial phase in the vaginal environment has been found to regulate the physiological activity of host cells. Studies have demonstrated that abnormal microbial growth in the vagina and a significant reduction in the proportion of lactic acid bacteria promote the occurrence of spontaneous preterm birth (sPTB). However, the contributing mechanism remains unknown. (2) Methods: This study uses extracellular vesicles (EVs) secreted by the probiotic Lactobacillus crispatus, commonly found in the vagina, to explore their potential to attenuate placental cells caused by oxidative stress induction. (3) Results: We found that L. crispatus-derived EVs improved Akt phosphorylation and attenuated both cell senescence and death in placental cells caused by oxidative stress induction. In addition, L. crispatus-derived EVs enhanced the resistance to H2O2 induction mediated by increasing mitochondrial fusion. (4) Conclusion: This is the first study to demonstrate that L. crispatus in the vagina can not only regulate the physiological functions of placental cells through the delivery of L. crispatus-EVs but also reduce cell senescence. As cell senescence is related to the occurrence of sPTB, these results indicate that maintaining the population of L. crispatus in the vaginal environment should be an adjuvant treatment strategy to avoid sPTB.
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页数:11
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