Optimization of data collection taking radiation damage into account

被引：110
作者：
Bourenkov, Gleb P. [2 ]
Popov, Alexander N. [1 ]
机构：
[1] European Synchrotron Radiat Facil, F-38043 Grenoble, France
[2] DESY, EMBL Hamburg Outstn, D-22607 Hamburg, Germany
来源：
ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY | 2010年 / 66卷
关键词：
X-RAY-DIFFRACTION; MACROMOLECULAR CRYSTALS; PROTEIN CRYSTALS; RECONSTRUCTION; ABSORPTION; SIMULATION;
D O I：
10.1107/S0907444909054961
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
To take into account the effects of radiation damage, new algorithms for the optimization of data-collection strategies have been implemented in the software package BEST. The intensity variation related to radiation damage is approximated by log-linear functions of resolution and cumulative X-ray dose. Based on an accurate prediction of the basic characteristics of data yet to be collected, BEST establishes objective relationships between the accessible data completeness, resolution and signal-to-noise statistics that can be achieved in an experiment and designs an optimal plan for data collection.
引用
收藏
页码：409 / 419
页数：11
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