Tubeless biochip for tailoring cell co-cultures in closed microchambers

被引：0

作者：

Meucci, Sandro ^{[1
,2
,3
]}

Vittorio, Orazio ^{[1
,2
,3
]}

Beltram, Fabio ^{[1
,2
,3
]}

Cecchini, Marco ^{[1
,2
]}

机构：

[1] Scuola Normale Super Pisa, NEST, I-56127 Pisa, Italy

[2] CNR, Ist Nanosci, I-56127 Pisa, Italy

[3] Ist Italian Tecnol, Ctr Nanotechnol Innovat NEST, I-56127 Pisa, Italy

来源：

MICROELECTRONIC ENGINEERING | 2014年 / 124卷

关键词：

Microfluidics; Co-cultures; Tubeless; Microchamber; Migration; Cell filter; GUIDANCE; SYSTEM;

D O I：

10.1016/j.mee.2014.04.015

中图分类号：

TM [电工技术]; TN [电子技术、通信技术];

学科分类号：

0808 ; 0809 ;

摘要：

Lab-on-a-Chip devices exploit micro/nanoengineering techniques to develop miniaturized systems for handling and manipulating small volumes of fluids. Here, we demonstrate a microfluidic device for culturing cells in closed microchambers that is designed to be compatible with standard biological procedures. This approach facilitates the thermal and gaseous equilibration of all the components, preventing the nucleation of air bubbles. Serial asymmetrical loadings were performed to realize co-cultures with initial topographical organization. The migration of two different cell types was monitored for 72 h by high-resolution fluorescence imaging. (C) 2014 Elsevier B.V. All rights reserved.

引用

页码：8 / 12

页数：5

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