Analytical strategy for determination of known and unknown destruxins using hybrid quadrupole-Orbitrap high-resolution mass spectrometry

被引：12

作者：

Arroyo-Manzanares, Natalia ^{[1
,2
]}

Di Mavungu, Jose Diana ^{[1
]}

Garrido-Jurado, Inmaculada ^{[3
]}

Arce, Lourdes ^{[2
]}

Vanhaecke, Lynn ^{[4
]}

Quesada-Moraga, Enrique ^{[3
]}

De Saeger, Sarah ^{[1
]}

机构：

[1] Univ Ghent, Fac Pharmaceut Sci, Lab Food Anal, Ottergemsesteenweg 460, B-9000 Ghent, Belgium

[2] Univ Cordoba, Dept Analyt Chem, Annex C3 Bldg,Campus Rabanales, Cordoba 14071, Spain

[3] Univ Cordoba, Dept Agr & Forestry Sci, ETSIAM, Bldg C4,Campus Rabanales, Cordoba 14071, Spain

[4] Univ Ghent, Dept Vet Publ Hlth & Food Safety, Lab Chem Anal, Fac Vet Med, Salisburylaan 133, B-9820 Merelbeke, Belgium

来源：

ANALYTICAL AND BIOANALYTICAL CHEMISTRY | 2017年 / 409卷 / 13期

关键词：

Destruxins identification; Untargeted analysi; Hybrid quadrupole-Orbitrap; Fragmentation pathway; METARHIZIUM-ANISOPLIAE; FUNGUS; DEPSIPEPTIDES;

D O I：

10.1007/s00216-017-0276-z

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

An analytical strategy based on a hybrid quadrupole-Orbitrap mass spectrometry was proposed for the simultaneous screening of known destruxins and characterization of potential members of this class of secondary metabolites, in order to evaluate the metabolite production of entomopathogenic fungi used as biocontrol agents. Initially, the fragmentation pathway of the known and commercially available destruxin A was established combining high resolution mass spectrometry (HRMS) and multiple stage MS data in order to obtain the strategy for the characterization of other destruxins for which reference standards were not available. Nineteen known destruxins including A, B, C, D, Ed, F, A(1), B-1, Ed(1), A(2), B-2, D-2, A(3), DesmA, DesmB, DesmC, DesmB(2), and two chloro-derivatives (Cl and E-2 chlorohydrin) were unequivocally identified in Metarhizium brunneum using the proposed strategy. In addition, four unknown destruxins, namely C-1, Ed(2), G, and G(1), were structurally elucidated and characterized for the first time in this fungal strain.

引用

页码：3347 / 3357

页数：11

共 24 条

[1] Use of UHPLC high-resolution Orbitrap mass spectrometry to investigate the genes involved in the production of secondary metabolites in Aspergillus flavus [J].