Phylogenetic and Transcription Analysis of Chrysanthemum WRKY Transcription Factors

被引:41
作者
Song, Aiping [1 ,2 ]
Li, Peiling [1 ,2 ]
Jiang, Jiafu [1 ]
Chen, Sumei [1 ]
Li, Huiyun [1 ]
Zeng, Jun [1 ]
Shao, Yafeng [1 ]
Zhu, Lu [1 ]
Zhang, Zhaohe [1 ]
Chen, Fadi [1 ,2 ]
机构
[1] Nanjing Agr Univ, Coll Hort, Nanjing 210095, Jiangsu, Peoples R China
[2] Jiangsu Prov Engn Lab Modern Facil Agr Technol &, Nanjing 210095, Jiangsu, Peoples R China
关键词
Chrysanthemum morifolium; phylogenetic analysis; stress response; transcription pattern; GENE-EXPRESSION; SALICYLIC-ACID; ARABIDOPSIS; DEFENSE; RESISTANCE; DROUGHT; IDENTIFICATION; REGULATORS; SENESCENCE; TOLERANCE;
D O I
10.3390/ijms150814442
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
WRKY transcription factors are known to function in a number of plant processes. Here we have characterized 15 WRKY family genes of the important ornamental species chrysanthemum (Chrysanthemum morifolium). A total of 15 distinct sequences were isolated; initially internal fragments were amplified based on transcriptomic sequence, and then the full length cDNAs were obtained using RACE (rapid amplification of cDNA ends) PCR. The transcription of these 15 genes in response to a variety of phytohormone treatments and both biotic and abiotic stresses was characterized. Some of the genes behaved as would be predicted based on their homology with Arabidopsis thaliana WRKY genes, but others showed divergent behavior.
引用
收藏
页码:14442 / 14455
页数:14
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