High-resolution atomic force microscopy of duplex and triplex DNA molecules

被引：45
作者：
Klinov, Dmitry
Dwir, Benjamin
Kapon, Eli
Borovok, Natalia
Molotsky, Tatiana
Kotlyar, Alexander
机构：
[1] Ecole Polytech Fed Lausanne, Lab Phys Nanostruct, CH-1015 Lausanne, Switzerland
[2] Russian Acad Sci, Shemyakin Ovchinnikov Inst Bioorgan Chem, Moscow, Russia
[3] Tel Aviv Univ, George S Wise Fac Life Sci, Dept Biochem, IL-69978 Tel Aviv, Israel
[4] Tel Aviv Univ, Inst Nanosci, IL-69978 Tel Aviv, Israel
来源：
NANOTECHNOLOGY | 2007年 / 18卷 / 22期
关键词：
D O I：
10.1088/0957-4484/18/22/225102
中图分类号：
TB3 [工程材料学];
学科分类号：
0805 ; 080502 ;
摘要：
Double-stranded poly(dG) - poly(dC) and triple-stranded poly( dG) - poly( dG) - poly( dC) DNA were deposited on the modified surface of highly oriented pyrolitic graphite ( HOPG) and visualized using atomic force microscopy with high-resolution ( radius of similar to 1 nm) tips. The high resolution attained by this technique enabled us to detect single-stranded regions in double-stranded poly( dG) - poly( dC) and double-stranded and single-stranded regions in poly( dG) - poly( dG) - poly( dC) triplexes, as well as to resolve the helical pitch of the triplex molecules. We could also follow the reaction of G-strand extension in poly( dG) - poly( dC) by the Klenow exo(-) fragment of DNA polymerase I. This approach to molecular visualization could serve as a useful tool for the investigation of irregular structures in canonical DNA and other biopolymers, as well as studies of the molecular mechanisms of DNA replication and transcription.
引用
收藏
页数：8
相关论文
共 22 条
[1]   Observation of single-stranded DNA on mica and highly oriented pyrolytic graphite by atomic force microscopy [J].
Adamcik, Jozef ;
Klinov, Dmitry V. ;
Witz, Guillaume ;
Sekatskii, Sergey K. ;
Dietler, Giovanni .
FEBS LETTERS, 2006, 580 (24) :5671-5675
[2]   ATOMIC FORCE MICROSCOPE [J].
BINNIG, G ;
QUATE, CF ;
GERBER, C .
PHYSICAL REVIEW LETTERS, 1986, 56 (09) :930-933
[3]   Estimating the persistence length of a worm-like chain molecule from force-extension measurements [J].
Bouchiat, C ;
Wang, MD ;
Allemand, JF ;
Strick, T ;
Block, SM ;
Croquette, V .
BIOPHYSICAL JOURNAL, 1999, 76 (01) :409-413
[4]   TRIPLEX DNA STRUCTURES [J].
FRANKKAMENETSKII, MD ;
MIRKIN, SM .
ANNUAL REVIEW OF BIOCHEMISTRY, 1995, 64 :65-95
[5]   THE STRUCTURE OF SODIUM THYMONUCLEATE FIBRES .1. THE INFLUENCE OF WATER CONTENT [J].
FRANKLIN, RE ;
GOSLING, RG .
ACTA CRYSTALLOGRAPHICA, 1953, 6 (8-9) :673-677
[6]   MOLECULAR CONFIGURATION OF DEOXYRIBONUCLEIC ACID .4. X-RAY DIFFRACTION STUDY OF A FORM [J].
FULLER, W ;
WILKINS, MHF ;
WILSON, HR ;
HAMILTON, LD .
JOURNAL OF MOLECULAR BIOLOGY, 1965, 12 (01) :60-&
[7]   CHARACTERIZATION OF CARBON NANOTUBES BY SCANNING PROBE MICROSCOPY [J].
GALLAGHER, MJ ;
CHEN, D ;
JACOBSEN, BP ;
SARID, D ;
LAMB, LD ;
TINKER, FA ;
JIAO, J ;
HUFFMAN, DR ;
SERAPHIN, S ;
ZHOU, D .
SURFACE SCIENCE, 1993, 281 (03) :L335-L340
[8]   Atomic force microscopy of long and short double-stranded, single-stranded and triple-stranded nucleic acids [J].
Hansma, HG ;
Revenko, I ;
Kim, K ;
Laney, DE .
NUCLEIC ACIDS RESEARCH, 1996, 24 (04) :713-720
[9]   IMMOBILIZING DNA ON GOLD VIA THIOL MODIFICATION FOR ATOMIC-FORCE MICROSCOPY IMAGING IN BUFFER SOLUTIONS [J].
HEGNER, M ;
WAGNER, P ;
SEMENZA, G .
FEBS LETTERS, 1993, 336 (03) :452-456
[10]   Thickness and low-temperature conductivity of DNA molecules [J].
Kasumov, AY ;
Klinov, DV ;
Roche, PE ;
Guéron, S ;
Bouchiat, H .
APPLIED PHYSICS LETTERS, 2004, 84 (06) :1007-1009
123