Evidence of co-exposure with Brucella spp, Coxiella burnetii, and Rift Valley fever virus among various species of wildlife in Kenya

被引：11

作者：

Gakuya, Francis ^{[1
]}

Akoko, James ^{[2
,3
]}

Wambua, Lillian ^{[2
]}

Nyamota, Richard ^{[2
]}

Ronoh, Bernard ^{[4
]}

Lekolool, Isaac ^{[4
]}

Mwatondo, Athman ^{[2
,5
,6
]}

Muturi, Mathew ^{[2
,5
,7
]}

Ouma, Collins ^{[3
]}

Nthiwa, Daniel ^{[8
]}

Middlebrook, Earl ^{[9
]}

Fair, Jeanne ^{[9
]}

Gachohi, John ^{[10
,11
]}

Njenga, Kariuki ^{[10
]}

Bett, Bernard ^{[2
]}

机构：

[1] Wildlife Res & Training Inst, Naivasha, Kenya

[2] Int Livestock Res Inst, Nairobi, Kenya

[3] Maseno Univ, Dept Biomed Sci, Kisumu, Kenya

[4] Kenya Wildlife Serv, Nairobi, Kenya

[5] Zoonot Dis Unit, Nairobi, Kenya

[6] Univ Nairobi, Fac Hlth, Dept Med Microbiol & Immunol, Nairobi, Kenya

[7] Free Univ Berlin, Fac Vet Med, Berlin, Germany

[8] Univ Embu, Dept Biol Sci, Embu, Kenya

[9] Los Alamos Natl Lab, Los Alamos, NM USA

[10] Washington State Univ, Global Hlth Programme, Nairobi, Kenya

[11] Jomo Kenyatta Univ Agr & Technol, Sch Publ Hlth, Nairobi, Kenya

来源：

PLOS NEGLECTED TROPICAL DISEASES | 2022年 / 16卷 / 08期

关键词：

PREVALENCE; COMMUNITY; DISEASES; ECOLOGY; CATTLE;

D O I：

10.1371/journal.pntd.0010596

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Background Co-infection, especially with pathogens of dissimilar genetic makeup, may result in a more devastating impact on the host. Investigations on co-infection with neglected zoonotic pathogens in wildlife are necessary to inform appropriate prevention and control strategies to reduce disease burden in wildlife and the potential transmission of these pathogens between wildlife, livestock and humans. This study assessed co-exposure of various Kenyan wildflife species with Brucella spp, Coxiella burnetii and Rift Valley fever virus (RVFV). Methodology A total of 363 sera from 16 different wildlife species, most of them (92.6%) herbivores, were analysed by Enzyme-linked immunosorbent assay (ELISA) for IgG antibodies against Brucella spp, C. burnetii and RVFV. Further, 280 of these were tested by PCR to identify Brucella species. Results Of the 16 wildlife species tested, 15 (93.8%) were seropositive for at least one of the pathogens. Mean seropositivities were 18.9% (95% CI: 15.0-23.3) for RVFV, 13.7% (95% CI: 10.3-17.7) for Brucella spp and 9.1% (95% CI: 6.3-12.5) for C. burnetii. Buffaloes (n = 269) had higher seropositivity for Brucella spp. (17.1%, 95% CI: 13.0-21.7%) and RVFV (23.4%, 95% CI: 18.6-28.6%), while giraffes (n = 36) had the highest seropositivity for C. burnetii (44.4%, 95% CI: 27.9-61.9%). Importantly, 23 of the 93 (24.7%) animals positive for at least one pathogen were co-exposed, with 25.4% (18/71) of the positive buffaloes positive for brucellosis and RVFV. On molecular analysis, Brucella DNA was detected in 46 (19.5%, CI: 14.9-24.7) samples, with 4 (8.6%, 95% CI: 2.2-15.8) being identified as B. melitensis. The Fisher's Exact test indicated that seropositivity varied significantly within the different animal families, with Brucella (p = 0.013), C. burnetii (p = < 0.001) and RVFV (p = 0.007). Location was also significantly associated (p = <0.001) with Brucella spp. and C. burnetii seropositivities. Conclusion Of similar to 20% of Kenyan wildlife that are seropositive for Brucella spp, C. burnetii and RVFV, almost 25% indicate co-infections with the three pathogens, particularly with Brucella spp and RVFV.

引用

页数：14

共 44 条

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