The biliprotein C-phycocyanin modulates the early radiation response: A pilot study

被引：15

作者：

Ivanova, Katia G. ^{[1
]}

Stankova, Katia G. ^{[1
]}

Nikolov, Vladimir N. ^{[1
]}

Georgieva, Radostina T. ^{[1
]}

Minkova, Kaledona M. ^{[2
]}

Gigova, Lili G. ^{[2
]}

Rupova, Ivanka T. ^{[1
]}

Boteva, Rayna N. ^{[1
]}

机构：

[1] Natl Ctr Radiobiol & Radiat Protect, Sofia 1756, Bulgaria

[2] Bulgarian Acad Sci, Inst Plant Physiol, BU-1113 Sofia, Bulgaria

来源：

MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS | 2010年 / 695卷 / 1-2期

关键词：

Antioxidants; ROS; Low-dose radiation; C-phycocyanin; Manganese super oxide dismutase (MnSOD); Catalase; Glutathione-S-transferase (GST); Occupational exposure; NF-KAPPA-B; ADAPTIVE RESPONSE; ANTIOXIDANT ENZYMES; IONIZING-RADIATION; OXIDATIVE STRESS; DAMAGE; DNA;

D O I：

10.1016/j.mrgentox.2009.11.002

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

This study analyzed the effects of biliprotein C-phycocyanin (C-PC) on the enzymatic antioxidant defence system in lymphocytes of nuclear power-plant workers and non-exposed controls. Changes in the protein levels of manganese super oxide dismutase (MnSOD), catalase and glutathione-S-transferase (GST) were used as markers for early biological effects of a single in vitro exposure of cells to: (i) 2 Gy gamma rays; (ii) 5 mu M C-PC: and (iii) a combination of C-PC plus irradiation with 2 Gy. The results showed that C-PC selectively stimulated the lymphocyte antioxidant defence system of occupationally exposed subjects. The activation of the antioxidant protective mechanisms as part of the early radiation response was probably related to the chronic low-dose occupational exposure. The modulating capacity of C-PC at the molecular level may be of interest for the protection of occupationally exposed persons. (C) 2009 Elsevier B.V. All rights reserved.

引用

页码：40 / 45

页数：6

共 29 条

[1] Urokinase-type plasminogen activator potentiates lipopolysaccharide-induced neutrophil activation [J].

Abraham, E ;

Gyetko, MR ;

Kuhn, K ;

Arcaroli, J ;

Strassheim, D ;

Park, JS ;

Shetty, S ;

Idell, S .

JOURNAL OF IMMUNOLOGY, 2003, 170 (11) :5644-5651

[2] NF-κB-mediated adaptive resistance to ionizing radiation [J].

Ahmed, Kazi Mokim ;

Li, Jian Jian .

FREE RADICAL BIOLOGY AND MEDICINE, 2008, 44 (01) :1-13

[3] Purification and characterization of phycocyanin from the blue-green alga Aphanizomenon flos-aquae [J].

Benedetti, S ;

Rinalducci, S ;

Benvenuti, F ;

Francogli, S ;

Pagliarani, S ;

Giorgi, L ;

Micheloni, M ;

D'Amici, GM ;

Zolla, L ;

Canestrari, F .

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2006, 833 (01) :12-18

[4]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[5]

Bravard A, 1999, INT J RADIAT BIOL, V75, P639

[6] Pre-exposure to low doses: Modulation of x-ray-induced DNA damage and repair? [J].

Cramers, P ;

Atanasova, P ;

Vrolijk, H ;

Darroudi, F ;

van Zeeland, AA ;

Huiskamp, R ;

Mullenders, LHF ;

Kleinjans, JCS .

RADIATION RESEARCH, 2005, 164 (04) :383-390

[7] Free radicals in the physiological control of cell function [J].

Dröge, W .

PHYSIOLOGICAL REVIEWS, 2002, 82 (01) :47-95

[8] Antioxidant properties of recombinant allophycocyanin expressed in Escherichia coli [J].

Ge, Baosheng ;

Qin, Song ;

Han, Lu ;

Lin, Fan ;

Ren, Yuhong .

JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY, 2006, 84 (03) :175-180

[9]

Greenberger JS, 2007, IN VIVO, V21, P141

[10] Manganese superoxide dismutase-mediated gene expression in radiation-induced adaptive responses [J].

Guo, GZ ;

Yan-Sanders, Y ;

Lyn-Cook, BD ;

Wang, TL ;

Tamae, D ;

Ogi, J ;

Khaletskiy, A ;

Li, ZK ;

Weydert, C ;

Longmate, JA ;

Huang, TT ;

Spitz, DR ;

Oberley, LW ;

Li, JJ .

MOLECULAR AND CELLULAR BIOLOGY, 2003, 23 (07) :2362-2378

← 1 2 3 →