In vitro engineered cartilage using synovium-derived mesenchymal stem cells with injectable gellan hydrogels

被引：50
作者：
Fan, Jiabing [1 ,2 ]
Gong, Yihong [1 ]
Ren, Li [3 ]
Varshney, Rohan R. [1 ]
Cai, Daozhang [2 ]
Wang, Dong-An [1 ,3 ]
机构：
[1] Nanyang Technol Univ, Div BioEngn, Sch Chem & Biomed Engn, Singapore 637457, Singapore
[2] Sun Yat Sen Univ, Ctr Stem Cell Biol & Tissue, Guangzhou 510080, Guangdong, Peoples R China
[3] S China Univ Technol, Minist Educ, Key Lab Specially Funct Mat & Adv Mfg Technol, Guangzhou 510641, Guangdong, Peoples R China
来源：
ACTA BIOMATERIALIA | 2010年 / 6卷 / 03期
关键词：
Synovium-derived mesenchymal stem cells; Gellan; Hydrogel; Injectability; Cartilage; CHONDROITIN SULFATE; BONE-MARROW; TISSUE; REPAIR; CHONDROGENESIS; COMPARTMENT; INTEGRATION; COMPOSITES; MEMBRANE; ALGINATE;
D O I：
10.1016/j.actbio.2009.08.042
中图分类号：
R318 [生物医学工程];
学科分类号：
0831 ;
摘要：
Synovium-derived mesenchymal stem cells (SMSC), a novel line of stem cells, are regarded as a promising cell Source for cartilage tissue engineering. The goal of this study was to investigate rabbit SMSC coupled with injectable gellan hydrogels for in vitro engineered cartilage SMSC were isolated from rabbit synovial tissue. amplified to passage 4 in monolayer, and encapsulated In injectable gellan hydrogels, constructs Of which were Cultured in chondrogenic medium supplemented with TGF-beta 1, TGF-beta 3 or BMP-2 for up to 42 days. The quality of the constructs was assessed in terms of cell proliferation and chondrocytic gene/protein expression using WST-1 assay, real-time RT-PCR, biochemical analysis, histology and immunohistochemical analysis Results indicate that the viability of SMSC in hydrogels treated with TGF-beta 1. TGF-beta 3 and BMP-2 remained high at culture time. The constructs formed cartilaginous tissue with the expression of chondrocytic genes (collagen type 11, aggrecan, biglycan. SOX 9) and cartilaginous matrix (sulphated glycosaminoglycan and collagen) as early as 21 days in culture. Both TGF-beta 1 and TGF-beta 3 treated SMSC-laden hydrogels showed more chondrogenesis compared with BMP-2 treated SMSC-laden hydrogels It demonstrates that injectable SMSC-laden gels, when treated with TGF-beta 1, TGF-beta 3 or BMP-2, are highly competent for in vitro engineered cartilage formation, which lays a foundation for their potential application in clinical cartilage repair (C) 2009 Acta Materialia Inc Published by Elsevier Ltd All rights reserved
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页码：1178 / 1185
页数：8
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