Efficient downregulation of alb1 gene using an AMA1-based episomal expression of RNAi construct in Aspergillus fumigatus

被引：16

作者：

Khalaj, Vahid

Eslami, Hamid

Azizi, Mohammad

Rovira-Graells, Nuria

Bromley, Mike

机构：

[1] Pasteur Inst Iran, Biotechnol Res Ctr, Tehran 13164, Iran

[2] F2g Ltd, Manchester, Lancs, England

来源：

FEMS MICROBIOLOGY LETTERS | 2007年 / 270卷 / 02期

关键词：

AMA1; RNAi; Aspergillus; cbhB;

D O I：

10.1111/j.1574-6968.2007.00680.x

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

An episomal RNAi silencing construct containing the inducible cbhB promoter and a hairpin structure has been made to downregulate the alb1 gene in the human pathogen Aspergillus fumigatus. Transformation of fungal protoplasts resulted in a high number of transformants with an inducible silenced phenotype (white spores). Efficient downregulation of the alb1 gene using this system suggests that this approach may overcome the variable downregulation observed with integrative constructs.

引用

收藏

页码：250 / 254

页数：5

相关论文

共 15 条

[1] Autonomous plasmid replication in Aspergillus nidulans: AMA1 and MATE elements [J].

Aleksenko, A ;

Clutterbuck, AJ .

FUNGAL GENETICS AND BIOLOGY, 1997, 21 (03) :373-387

[2] Gene expression from replicating plasmids in Aspergillus nidulans [J].

Aleksenko, A ;

Nikolaev, I ;

Vinetski, Y ;

Clutterbuck, AJ .

MOLECULAR AND GENERAL GENETICS, 1996, 253 (1-2) :242-246

[3] RECOMBINATIONAL STABILITY OF REPLICATING PLASMIDS IN ASPERGILLUS-NIDULANS DURING TRANSFORMATION, VEGETATIVE GROWTH AND SEXUAL REPRODUCTION [J].

ALEKSENKO, AY ;

CLUTTERBUCK, AJ .

CURRENT GENETICS, 1995, 28 (01) :87-93

[4] The Aspergillus fumigatus cellobiohydrolase B (cbhB) promoter is tightly regulated and can be exploited for controlled protein expression and RNAi [J].

Bromley, Michael ;

Gordon, Caroline ;

Rovira-Graells, Nuria ;

Oliver, Jason .

FEMS MICROBIOLOGY LETTERS, 2006, 264 (02) :246-254

[5] RNA-mediated gene silencing in monokaryons and dikaryons of Schizophyllum commune [J].

de Jong, JF ;

Deelstra, HJ ;

Wösten, HAB ;

Lugones, LG .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2006, 72 (02) :1267-1269

[6] Simultaneous silencing of multiple genes in the apple scab fungus, Venturia inaequalis, by expression of RNA with chimeric inverted repeats [J].

Fitzgerald, A ;

van Kan, JAL ;

Plummer, KM .

FUNGAL GENETICS AND BIOLOGY, 2004, 41 (10) :963-971

[7] Gene silencing with RNA interference in the human pathogenic fungus Aspergillus fumigatus [J].

Mouyna, I ;

Henry, C ;

Doering, TL ;

Latgé, JP .

FEMS MICROBIOLOGY LETTERS, 2004, 237 (02) :317-324

[8] RNA silencing in fungi: Mechanisms and applications [J].

Nakayashiki, H .

FEBS LETTERS, 2005, 579 (26) :5950-5957

[9] RNA silencing as a tool for exploring gene function in ascomycete fungi [J].

Nakayashiki, H ;

Hanada, S ;

Quoc, NB ;

Kadotani, N ;

Tosa, Y ;

Mayama, S .

FUNGAL GENETICS AND BIOLOGY, 2005, 42 (04) :275-283

[10] Genomic sequence of the pathogenic and allergenic filamentous fungus Aspergillus fumigatus [J].

Nierman, WC ;

Pain, A ;

Anderson, MJ ;

Wortman, JR ;

Kim, HS ;

Arroyo, J ;

Berriman, M ;

Abe, K ;

Archer, DB ;

Bermejo, C ;

Bennett, J ;

Bowyer, P ;

Chen, D ;

Collins, M ;

Coulsen, R ;

Davies, R ;

Dyer, PS ;

Farman, M ;

Fedorova, N ;

Fedorova, N ;

Feldblyum, TV ;

Fischer, R ;

Fosker, N ;

Fraser, A ;

García, JL ;

García, MJ ;

Goble, A ;

Goldman, GH ;

Gomi, K ;

Griffith-Jones, S ;

Gwilliam, R ;

Haas, B ;

Haas, H ;

Harris, D ;

Horiuchi, H ;

Huang, J ;

Humphray, S ;

Jiménez, J ;

Keller, N ;

Khouri, H ;

Kitamoto, K ;

Kobayashi, T ;

Konzack, S ;

Kulkarni, R ;

Kumagai, T ;

Lafton, A ;

Latgé, JP ;

Li, WX ;

Lord, A ;

Majoros, WH .

NATURE, 2005, 438 (7071) :1151-1156